Adenovirus-mediated gene transfer of B7.1 induces immunological anti-tumor effects in a murine brain tumor

Jun Morioka, Koji Kajiwara, Koichi Yoshikawa, Makoto Ideguchi, Tetsuya Uchida, Yoshinori Ohmoto, Michiyasu Suzuki

研究成果: Article査読

9 被引用数 (Scopus)

抄録

The purpose of the present study was to determine if adenovirus-mediated transfection of a syngeneic mouse brain tumor with the gene encoding B7.1 enhances immunogenicity against tumor. Malignant astrocytoma cells were transfected with adenoviral vectors carrying the B7.1 gene (AdB7). Immunocytochemical analysis confirmed the expression of B7.1 in vitro and in vivo. To investigate the effects of B7.1 expression on tumorigenicity of the malignant astrocytoma, mice were implanted intracerebrally with B7.1-transfected glioma cells. There was no significant difference in proliferation between B7.1-transfected cells and controls in vitro. Nevertheless, mice implanted with B7.1-transfected cells survived significantly longer than those in the control groups. Immunocytochemical analysis of the tumors showed that there was infiltration of a number of CD8+ T-cells and CD25+ activated T-cells in the brain implanted with B7.1-transfected glioma cells. The results showed the possibility that adenovirus-mediated B7.1 gene transfection to a brain tumor induced activation of CD8+ cytotoxic T-lymphocytes.

本文言語English
ページ(範囲)13-23
ページ数11
ジャーナルJournal of Neuro-Oncology
60
1
DOI
出版ステータスPublished - 01-10-2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • Oncology
  • Neurology
  • Clinical Neurology
  • Cancer Research

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