Adhesion via CD43 induces Syk activation and cell proliferation in TF-1 cells

Yasuo Miura, Chisato Mizutani, Toshio Nishihara, Terutoshi Hishita, Shigeru Yanagi, Yumi Tohyama, Satoshi Ichiyama, Hirohei Yamamura, Takashi Uchiyama, Kaoru Tohyama

研究成果: Article査読

10 被引用数 (Scopus)


The effect of adhesion via CD43 (leukosialin, sialophorin) on cell proliferation and phosphorylation signaling were examined in a growth factor-dependent hematopoietic progenitor cell line, TF-1. TF-1 cells promptly resulted in death after withdrawal of growth factors. However, the viable cell number increased when TF-1 cells were cultured on anti-CD43 monoclonal antibody-coated plates. In this case, sustained activation of protein tyrosine kinase Syk and extracellular signal-regulated kinase (Erk) 1/2 were detected. Overexpression of exogenous Syk on TF-1 cells by the adenovirus vector system induced enhancement of the cell proliferation accompanied with enhancement of the Erk activation by a dominant-positive effect. The signal transducer and activator of transcription (STAT) 5 seemed not to be associated with the CD43-mediated cell proliferation. These results indicated that adhesion via CD43 induces the proliferation of TF-1 cells in the absence of growth factors in part by Syk-dependent Erk 1/2 signaling.

ジャーナルBiochemical and Biophysical Research Communications
出版ステータスPublished - 19-10-2001

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学


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