Adipocyte-derived plasma protein, adiponectin, suppresses lipid accumulation and class A scavenger receptor expression in human monocyte-derived macrophages

Noriyuki Ouchi, Shinji Kihara, Yukio Arita, Makoto Nishida, Akifumi Matsuyama, Yoshihisa Okamoto, Masato Ishigami, Hiroshi Kuriyama, Ken Kishida, Hitoshi Nishizawa, Kikuko Hotta, Masahiro Muraguchi, Yasukazu Ohmoto, Shizuya Yamashita, Tohru Funahashi, Yuji Matsuzawa

研究成果: Article査読

1171 被引用数 (Scopus)


Background - Excessive lipid accumulation in macrophages plays an important role in the development of atherosclerosis. Recently, we discovered an adipocyte-specific plasma protein, adiponectin, that is decreased in patients with coronary artery, disease. We previously demonstrated that adiponectin acts as a modulator for pro-inflammatory stimuli and inhibits monocyte adhesion to endothelial cells. The present study investigated the effects of adiponectin on lipid accumulation in human monocyte-derived macrophages. Methods and Results - Human monocytes were differentiated into macrophages by incubation in human type AB serum for 7 days, and the effects of adiponectin were investigated at different time intervals. Treatment with physiological concentrations of adiponectin reduced intracellular cholesteryl ester content, as determined using the enzymatic, fluorometric method. The adiponectin-treated macrophages contained fewer lipid droplets stained by oil red O. Adiponectin suppressed the expression of the class A macrophage scavenger receptor (MSR) at both mRNA and protein levels by Northern and immunoblot analyses, respectively, without affecting the expression of CD36, which was quantified by flow cytometry. Adiponectin reduced the class A MSR promoter activity, as measured by luciferase reporter assay. Adiponectin treatment dose-dependently decreased class A MSR ligand binding and uptake activities. The mRNA level of lipoprotein lipase as a marker of macrophage differentiation was decreased by adiponectin treatment, but that of apolipoprotein E was not altered. Adiponectin was detected around macrophages in the human injured aorta by immunohistochemistry. Conclusions - The adipocyte-derived plasma protein adiponectin suppressed macrophage-to-foam cell transformation, suggesting that adiponectin may act as a modulator for macrophage-to-foam cell transformation.

出版ステータスPublished - 27-02-2001

All Science Journal Classification (ASJC) codes

  • 循環器および心血管医学
  • 生理学(医学)


「Adipocyte-derived plasma protein, adiponectin, suppresses lipid accumulation and class A scavenger receptor expression in human monocyte-derived macrophages」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。