Adiponectin promotes migration activities of endothelial progenitor cells via Cdc42/Rac1

Nobuhisa Nakamura; Keiko Naruse; Takashi Matsuki; Yoji Hamada; Eitaro Nakashima; Hideki Kamiya; Tatsuaki Matsubara; Atsushi Enomoto; Masahide Takahashi; Yutaka Oiso; Jiro Nakamura

doi:10.1016/j.febslet.2009.07.011

Adiponectin promotes migration activities of endothelial progenitor cells via Cdc42/Rac1

Nobuhisa Nakamura
, Keiko Naruse
, Takashi Matsuki
, Yoji Hamada
, Eitaro Nakashima
, Hideki Kamiya
, Tatsuaki Matsubara
, Atsushi Enomoto
, Masahide Takahashi
, Yutaka Oiso
, Jiro Nakamura

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

51 被引用数 (Scopus)

抄録

Adiponectin has anti-atherosclerotic effects through its direct actions on vascular cells. The present study investigates the molecular mechanisms of adiponectin in the migration of endothelial progenitor cells (EPCs) which play an important role in neovascularization and re-endothelization. The phosphorylation of Akt and the activations of Cdc42 and Rac1 were significantly increased by adiponectin. Adiponectin increased the migration activity of EPCs, which was completely inhibited by a PI3-kinase inhibitor. siRNA of Cdc42 or Rac1 completely inhibited the adiponectin-induced migration, but siRNA of Akt had no effects, indicating that adiponectin promotes the migration activities of EPCs mainly through PI3-kinase/Cdc42/Rac1. Structured summary: MINT-7217629: PAK1 (uniprotkb:Q13153) physically interacts (MI:0914) with CDC42 (uniprotkb:P60953) by pull down (MI:0096). MINT-7217644: PAK1 (uniprotkb:Q13153) physically interacts (MI:0914) with Rac1 (uniprotkb:P63000) by pull down (MI:0096).

本文言語	英語
ページ（範囲）	2457-2463
ページ数	7
ジャーナル	FEBS Letters
巻	583
号	15
DOI	https://doi.org/10.1016/j.febslet.2009.07.011
出版ステータス	出版済み - 06-08-2009
外部発表	はい

All Science Journal Classification (ASJC) codes

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

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10.1016/j.febslet.2009.07.011

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@article{eae148d35bef4691b20f37f4c494500e,

title = "Adiponectin promotes migration activities of endothelial progenitor cells via Cdc42/Rac1",

abstract = "Adiponectin has anti-atherosclerotic effects through its direct actions on vascular cells. The present study investigates the molecular mechanisms of adiponectin in the migration of endothelial progenitor cells (EPCs) which play an important role in neovascularization and re-endothelization. The phosphorylation of Akt and the activations of Cdc42 and Rac1 were significantly increased by adiponectin. Adiponectin increased the migration activity of EPCs, which was completely inhibited by a PI3-kinase inhibitor. siRNA of Cdc42 or Rac1 completely inhibited the adiponectin-induced migration, but siRNA of Akt had no effects, indicating that adiponectin promotes the migration activities of EPCs mainly through PI3-kinase/Cdc42/Rac1. Structured summary: MINT-7217629: PAK1 (uniprotkb:Q13153) physically interacts (MI:0914) with CDC42 (uniprotkb:P60953) by pull down (MI:0096). MINT-7217644: PAK1 (uniprotkb:Q13153) physically interacts (MI:0914) with Rac1 (uniprotkb:P63000) by pull down (MI:0096).",

author = "Nobuhisa Nakamura and Keiko Naruse and Takashi Matsuki and Yoji Hamada and Eitaro Nakashima and Hideki Kamiya and Tatsuaki Matsubara and Atsushi Enomoto and Masahide Takahashi and Yutaka Oiso and Jiro Nakamura",

note = "Funding Information: We thank Prof. Toyoaki Murohara for helpful discussions and technical assistance and Ms. Yuko Maehata and Ms. Keiko Shimamoto for excellent technical assistance. We also thank the cord blood donors and Dr. Osamu Narita, Dr. Sentoyo Kato, Dr. Chikako Ito, and the staffs of Narita Hospital and Chubu Rosai Hospital (Nagoya, Japan) for help in collecting the cord blood. This research was supported in part by a Grant-in-Aid for Scientific Research on the Priority Areas “System cell engineering by multi-scale manipulation” from the Ministry of Education, Culture, Sports, Science and Technology of Japan and in part by the “AGU High-Tech Research Center” Project for Private Universities: matching fund subsidy from MEXT.",

year = "2009",

month = aug,

day = "6",

doi = "10.1016/j.febslet.2009.07.011",

language = "English",

volume = "583",

pages = "2457--2463",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "15",

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T1 - Adiponectin promotes migration activities of endothelial progenitor cells via Cdc42/Rac1

AU - Nakamura, Nobuhisa

AU - Naruse, Keiko

AU - Matsuki, Takashi

AU - Hamada, Yoji

AU - Nakashima, Eitaro

AU - Kamiya, Hideki

AU - Matsubara, Tatsuaki

AU - Enomoto, Atsushi

AU - Takahashi, Masahide

AU - Oiso, Yutaka

AU - Nakamura, Jiro

N1 - Funding Information: We thank Prof. Toyoaki Murohara for helpful discussions and technical assistance and Ms. Yuko Maehata and Ms. Keiko Shimamoto for excellent technical assistance. We also thank the cord blood donors and Dr. Osamu Narita, Dr. Sentoyo Kato, Dr. Chikako Ito, and the staffs of Narita Hospital and Chubu Rosai Hospital (Nagoya, Japan) for help in collecting the cord blood. This research was supported in part by a Grant-in-Aid for Scientific Research on the Priority Areas “System cell engineering by multi-scale manipulation” from the Ministry of Education, Culture, Sports, Science and Technology of Japan and in part by the “AGU High-Tech Research Center” Project for Private Universities: matching fund subsidy from MEXT.

PY - 2009/8/6

Y1 - 2009/8/6

N2 - Adiponectin has anti-atherosclerotic effects through its direct actions on vascular cells. The present study investigates the molecular mechanisms of adiponectin in the migration of endothelial progenitor cells (EPCs) which play an important role in neovascularization and re-endothelization. The phosphorylation of Akt and the activations of Cdc42 and Rac1 were significantly increased by adiponectin. Adiponectin increased the migration activity of EPCs, which was completely inhibited by a PI3-kinase inhibitor. siRNA of Cdc42 or Rac1 completely inhibited the adiponectin-induced migration, but siRNA of Akt had no effects, indicating that adiponectin promotes the migration activities of EPCs mainly through PI3-kinase/Cdc42/Rac1. Structured summary: MINT-7217629: PAK1 (uniprotkb:Q13153) physically interacts (MI:0914) with CDC42 (uniprotkb:P60953) by pull down (MI:0096). MINT-7217644: PAK1 (uniprotkb:Q13153) physically interacts (MI:0914) with Rac1 (uniprotkb:P63000) by pull down (MI:0096).

AB - Adiponectin has anti-atherosclerotic effects through its direct actions on vascular cells. The present study investigates the molecular mechanisms of adiponectin in the migration of endothelial progenitor cells (EPCs) which play an important role in neovascularization and re-endothelization. The phosphorylation of Akt and the activations of Cdc42 and Rac1 were significantly increased by adiponectin. Adiponectin increased the migration activity of EPCs, which was completely inhibited by a PI3-kinase inhibitor. siRNA of Cdc42 or Rac1 completely inhibited the adiponectin-induced migration, but siRNA of Akt had no effects, indicating that adiponectin promotes the migration activities of EPCs mainly through PI3-kinase/Cdc42/Rac1. Structured summary: MINT-7217629: PAK1 (uniprotkb:Q13153) physically interacts (MI:0914) with CDC42 (uniprotkb:P60953) by pull down (MI:0096). MINT-7217644: PAK1 (uniprotkb:Q13153) physically interacts (MI:0914) with Rac1 (uniprotkb:P63000) by pull down (MI:0096).

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UR - https://www.scopus.com/pages/publications/67849118458#tab=citedBy

U2 - 10.1016/j.febslet.2009.07.011

DO - 10.1016/j.febslet.2009.07.011

M3 - Article

C2 - 19596003

AN - SCOPUS:67849118458

SN - 0014-5793

VL - 583

SP - 2457

EP - 2463

JO - FEBS Letters

JF - FEBS Letters

IS - 15

ER -

Adiponectin promotes migration activities of endothelial progenitor cells via Cdc42/Rac1

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