Age-related attenuation of HSP47 heat response in fibroblasts

Osamu Miyaishi, Yoshitake Ito, Ken ichi Kozaki, Tuneko Sato, Hajime Takechi, Kazuhiro Nagata, Shinsuke Saga

研究成果: Article査読

28 被引用数 (Scopus)

抄録

The collagen-binding heat shock protein of molecular weight 47 000 (HSP47), resident in the endoplasmic reticulum (ER), is assumed to play a specific role as a molecular chaperon in the processing of procollagen molecules. The present investigation of age-related alteration in the HSP47 heat response in cultured murine and human fibroblasts revealed expression in cells with a low population doubling level (PDL) derived from young mice and people more inducible by heat treatment than those from older mice and people. On the other hand, cells with a high PDL showed a very low heat response in terms of HSP47 expression regardless of the donor age. Northern blot analysis of HSP47 m-RNA indicated that the age related attenuation of HSP47 expression was regulated by transcriptional mechanisms. Furthermore, immunofluorescent analysis using a monoclonal antibody against the carboxylterminal propeptide of type I procollagen revealed far greater retention of procollagen molecules in the ER lumen of cells from old persons than in those from young persons. This was particularly prominent in heat-treated cells from old persons, indicating the possibility that the observed decrease in HSP47 heat response might cause blockage of procollagen transport to the Golgi and therefore secretion.

本文言語English
ページ(範囲)213-226
ページ数14
ジャーナルMechanisms of Ageing and Development
77
3
DOI
出版ステータスPublished - 13-01-1995
外部発表はい

All Science Journal Classification (ASJC) codes

  • Ageing
  • Developmental Biology

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