ALK7 is a novel marker for adipocyte differentiation

Masahiro Kogame, Shinji Matsuo, Masashi Nakatani, Akira Kurisaki, Hiromu Nishitani, Kunihiro Tsuchida, Hiromu Sugino

研究成果: Article

16 引用 (Scopus)

抄録

Transforming growth factor-β (TGF-β) family members regulate a variety of cellular functions and play important roles in cell differentiation. Activin receptor-like kinase 7 (ALK7), a receptor for TGF-β family members, was initially cloned from rats as an orphan receptor and has been recently shown to be a type I receptor for nodal, activin B and activin AB. ALK7 is expressed not only in neurons, but also in insulin-producing islet β cells and white and brown adipose tissues; however, the specific functions of ALK7 in these tissues are not known. In order to test whether ALK7 is involved in adipocyte differentiation, we analyzed its expression during adipocyte differentiation. ALK7 expression was detected in the late phase of adipocyte differentiation by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and immunofluorescence staining in 3T3-L1 cells. We also detected the expression of ALK7 by RT-PCR in stromal vascular fraction (SVF) cells. These results indicated that ALK7 is a novel marker specifically expressed during the late phase of adipocyte differentiation. Furthermore, our results suggest the possible involvement of nodal or activin B in adipocyte differentiation.

元の言語English
ページ(範囲)238-245
ページ数8
ジャーナルJournal of Medical Investigation
53
発行部数3-4
DOI
出版物ステータスPublished - 01-08-2006

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Activin Receptors
Differentiation Antigens
Adipocytes
Polymerase chain reaction
RNA-Directed DNA Polymerase
Transforming Growth Factors
Reverse Transcriptase Polymerase Chain Reaction
Tissue
3T3-L1 Cells
White Adipose Tissue
Brown Adipose Tissue
Islets of Langerhans
Neurons
Fluorescent Antibody Technique
Blood Vessels
Rats
Cell Differentiation
Western Blotting
Insulin
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Kogame, Masahiro ; Matsuo, Shinji ; Nakatani, Masashi ; Kurisaki, Akira ; Nishitani, Hiromu ; Tsuchida, Kunihiro ; Sugino, Hiromu. / ALK7 is a novel marker for adipocyte differentiation. :: Journal of Medical Investigation. 2006 ; 巻 53, 番号 3-4. pp. 238-245.
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abstract = "Transforming growth factor-β (TGF-β) family members regulate a variety of cellular functions and play important roles in cell differentiation. Activin receptor-like kinase 7 (ALK7), a receptor for TGF-β family members, was initially cloned from rats as an orphan receptor and has been recently shown to be a type I receptor for nodal, activin B and activin AB. ALK7 is expressed not only in neurons, but also in insulin-producing islet β cells and white and brown adipose tissues; however, the specific functions of ALK7 in these tissues are not known. In order to test whether ALK7 is involved in adipocyte differentiation, we analyzed its expression during adipocyte differentiation. ALK7 expression was detected in the late phase of adipocyte differentiation by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and immunofluorescence staining in 3T3-L1 cells. We also detected the expression of ALK7 by RT-PCR in stromal vascular fraction (SVF) cells. These results indicated that ALK7 is a novel marker specifically expressed during the late phase of adipocyte differentiation. Furthermore, our results suggest the possible involvement of nodal or activin B in adipocyte differentiation.",
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Kogame, M, Matsuo, S, Nakatani, M, Kurisaki, A, Nishitani, H, Tsuchida, K & Sugino, H 2006, 'ALK7 is a novel marker for adipocyte differentiation', Journal of Medical Investigation, 巻. 53, 番号 3-4, pp. 238-245. https://doi.org/10.2152/jmi.53.238

ALK7 is a novel marker for adipocyte differentiation. / Kogame, Masahiro; Matsuo, Shinji; Nakatani, Masashi; Kurisaki, Akira; Nishitani, Hiromu; Tsuchida, Kunihiro; Sugino, Hiromu.

:: Journal of Medical Investigation, 巻 53, 番号 3-4, 01.08.2006, p. 238-245.

研究成果: Article

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AU - Tsuchida, Kunihiro

AU - Sugino, Hiromu

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