AMPA receptor activates a G-protein that suppresses a cGMP-gated current

Fusao Kawai, Peter Sterling

研究成果: Article査読

53 被引用数 (Scopus)

抄録

The AMPA receptor, ubiquitous in brain, is termed 'ionotropic' because it gates an ion channel directly. We found that an AMPA receptor can also modulate a G-protein to gate an ion channel indirectly. Glutamate applied to a retinal ganglion cell briefly suppresses the inward current through a cGMP- gated channel. AMPA and kainate also suppress the current, an effect that is blocked both by their general antagonist CNQX and also by the relatively specific AMPA receptor antagonist GYKI-52466. Neither NMDA nor agonists of metabotropic glutamate receptors are effective. The AMPA-induced suppression of the cGMP-gated current is blocked when the patch pipette includes GDP-β- S, whereas the suppression is irreversible when the pipette contains GTP-γ- S. This suggests a G-protein mediator, and, consistent with this, pertussis toxin blocks the current suppression. Nitric oxide (NO) donors induce the current suppressed by AMPA, and phosphodiesterase inhibitors prevent the suppression. Apparently, the AMPA receptor can exhibit a 'metabotropic' activity that allows it to antagonize excitation evoked by NO.

本文言語English
ページ(範囲)2954-2959
ページ数6
ジャーナルJournal of Neuroscience
19
8
DOI
出版ステータスPublished - 15-04-1999
外部発表はい

All Science Journal Classification (ASJC) codes

  • 神経科学(全般)

フィンガープリント

「AMPA receptor activates a G-protein that suppresses a cGMP-gated current」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル