An active Ac-like transposable element in teleost fish

Hiroshi Hori, Miho Suzuki, Hidehito Inagaki, Takahisa Oshima, Akihiko Koga

研究成果: Article

10 引用 (Scopus)

抄録

The i4/i4 genotype of the medakafish, Oryzias latipes, exhibits a quasi albino phenotype due to insertion of a novel transposable element, Tol2, into the tyrosinase gene. Tol2 is 4681 bp in length, has short inverted terminal repeats, and contains four ORFs with the potential to encode a transposase protein. Excision activity of the element has been detected by PCR analysis. Genomic Southern of the Tol2 element revealed that about 20 copies are present in the diploid genome. Dot-matrix comparisons of amino acid sequences of ORFs show relatively high similarity with transposases from Ac of maize, hobo of Drosophila, and Tam3 of snapdragon, which are all active transposable elements. Tol2 is thus concluded to be an active Ac-like transposable element probably encoding a transposase protein. It should therefore find application as a unique material for establishing a gene tagging system in fish.

元の言語English
ページ(範囲)206-207
ページ数2
ジャーナルJournal of Marine Biotechnology
6
発行部数4
出版物ステータスPublished - 01-12-1998
外部発表Yes

Fingerprint

Transposases
DNA Transposable Elements
Fish
Fishes
Genes
Open Reading Frames
Antirrhinum
Oryzias
Monophenol Monooxygenase
Terminal Repeat Sequences
Diploidy
Drosophila
Zea mays
Amino Acid Sequence
Proteins
Genotype
Genome
Phenotype
Amino Acids
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Hori, H., Suzuki, M., Inagaki, H., Oshima, T., & Koga, A. (1998). An active Ac-like transposable element in teleost fish. Journal of Marine Biotechnology, 6(4), 206-207.
Hori, Hiroshi ; Suzuki, Miho ; Inagaki, Hidehito ; Oshima, Takahisa ; Koga, Akihiko. / An active Ac-like transposable element in teleost fish. :: Journal of Marine Biotechnology. 1998 ; 巻 6, 番号 4. pp. 206-207.
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Hori, H, Suzuki, M, Inagaki, H, Oshima, T & Koga, A 1998, 'An active Ac-like transposable element in teleost fish', Journal of Marine Biotechnology, 巻. 6, 番号 4, pp. 206-207.

An active Ac-like transposable element in teleost fish. / Hori, Hiroshi; Suzuki, Miho; Inagaki, Hidehito; Oshima, Takahisa; Koga, Akihiko.

:: Journal of Marine Biotechnology, 巻 6, 番号 4, 01.12.1998, p. 206-207.

研究成果: Article

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