抄録
The i4/i4 genotype of the medakafish, Oryzias latipes, exhibits a quasi albino phenotype due to insertion of a novel transposable element, Tol2, into the tyrosinase gene. Tol2 is 4681 bp in length, has short inverted terminal repeats, and contains four ORFs with the potential to encode a transposase protein. Excision activity of the element has been detected by PCR analysis. Genomic Southern of the Tol2 element revealed that about 20 copies are present in the diploid genome. Dot-matrix comparisons of amino acid sequences of ORFs show relatively high similarity with transposases from Ac of maize, hobo of Drosophila, and Tam3 of snapdragon, which are all active transposable elements. Tol2 is thus concluded to be an active Ac-like transposable element probably encoding a transposase protein. It should therefore find application as a unique material for establishing a gene tagging system in fish.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 206-207 |
| ページ数 | 2 |
| ジャーナル | Journal of Marine Biotechnology |
| 巻 | 6 |
| 発行部数 | 4 |
| 出版物ステータス | Published - 01-12-1998 |
| 外部発表 | Yes |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
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An active Ac-like transposable element in teleost fish. / Hori, Hiroshi; Suzuki, Miho; Inagaki, Hidehito; Oshima, Takahisa; Koga, Akihiko.
：: Journal of Marine Biotechnology, 巻 6, 番号 4, 01.12.1998, p. 206-207.研究成果: Article
TY - JOUR
T1 - An active Ac-like transposable element in teleost fish
AU - Hori, Hiroshi
AU - Suzuki, Miho
AU - Inagaki, Hidehito
AU - Oshima, Takahisa
AU - Koga, Akihiko
PY - 1998/12/1
Y1 - 1998/12/1
N2 - The i4/i4 genotype of the medakafish, Oryzias latipes, exhibits a quasi albino phenotype due to insertion of a novel transposable element, Tol2, into the tyrosinase gene. Tol2 is 4681 bp in length, has short inverted terminal repeats, and contains four ORFs with the potential to encode a transposase protein. Excision activity of the element has been detected by PCR analysis. Genomic Southern of the Tol2 element revealed that about 20 copies are present in the diploid genome. Dot-matrix comparisons of amino acid sequences of ORFs show relatively high similarity with transposases from Ac of maize, hobo of Drosophila, and Tam3 of snapdragon, which are all active transposable elements. Tol2 is thus concluded to be an active Ac-like transposable element probably encoding a transposase protein. It should therefore find application as a unique material for establishing a gene tagging system in fish.
AB - The i4/i4 genotype of the medakafish, Oryzias latipes, exhibits a quasi albino phenotype due to insertion of a novel transposable element, Tol2, into the tyrosinase gene. Tol2 is 4681 bp in length, has short inverted terminal repeats, and contains four ORFs with the potential to encode a transposase protein. Excision activity of the element has been detected by PCR analysis. Genomic Southern of the Tol2 element revealed that about 20 copies are present in the diploid genome. Dot-matrix comparisons of amino acid sequences of ORFs show relatively high similarity with transposases from Ac of maize, hobo of Drosophila, and Tam3 of snapdragon, which are all active transposable elements. Tol2 is thus concluded to be an active Ac-like transposable element probably encoding a transposase protein. It should therefore find application as a unique material for establishing a gene tagging system in fish.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032461159&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0032461159
VL - 6
SP - 206
EP - 207
JO - Journal of Marine Biotechnology
JF - Journal of Marine Biotechnology
SN - 0941-2905
IS - 4
ER -