An active Ac-like transposable element in teleost fish

Hiroshi Hori, Miho Suzuki, Hidehito Inagaki, Takahisa Oshima, Akihiko Koga

研究成果: Article査読

10 被引用数 (Scopus)

抄録

The i4/i4 genotype of the medakafish, Oryzias latipes, exhibits a quasi albino phenotype due to insertion of a novel transposable element, Tol2, into the tyrosinase gene. Tol2 is 4681 bp in length, has short inverted terminal repeats, and contains four ORFs with the potential to encode a transposase protein. Excision activity of the element has been detected by PCR analysis. Genomic Southern of the Tol2 element revealed that about 20 copies are present in the diploid genome. Dot-matrix comparisons of amino acid sequences of ORFs show relatively high similarity with transposases from Ac of maize, hobo of Drosophila, and Tam3 of snapdragon, which are all active transposable elements. Tol2 is thus concluded to be an active Ac-like transposable element probably encoding a transposase protein. It should therefore find application as a unique material for establishing a gene tagging system in fish.

本文言語English
ページ(範囲)206-207
ページ数2
ジャーナルJournal of Marine Biotechnology
6
4
出版ステータスPublished - 01-12-1998
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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