An optimized protein extraction method for gel-free proteomic analysis of opuntia ficus-indica

Akiko Hashiguchi, Hisateru Yamaguchi, Keisuke Hitachi, Kazuo Watanabe

研究成果: ジャーナルへの寄稿学術論文査読

3 被引用数 (Scopus)


Opuntia spp. is an economically important vegetable crop with high stress-tolerance and health benefits. However, proteomic analysis of the plant has been difficult due to the composition of its succulent cladodes; the abundant polysaccharides interfere with protein extraction. To facilitate proteomic analysis of this plant, we present a rapid and simple protein extraction method for Opuntia ficus-indica (L.) Miller. The optimized method produced highly reproducible protein patterns and was compatible with a gel-free quantitative workflow without the need for additional purification. We successfully analyzed the cladode mesocarp and exocarp tissues, resulting in the identification of 319 proteins. In addition, we used this method to examine the relative changes in the Opuntia proteome in response to salt stress to determine whether physiological changes could be captured. Qualified observations were obtained, revealing that salt stress increased phosphoenolpyruvate carboxylase abundance and decreased ribulose-bisphosphate carboxylase in young O. ficus-indica plants. These findings suggest that Crassulacean acid metabolism is promoted under salinity. This study highlights the efficacy of our optimized protein extraction method for elucidating the metabolic adaptations of Opuntia using gel-free proteomic analysis.

出版ステータス出版済み - 01-2021

All Science Journal Classification (ASJC) codes

  • 生態、進化、行動および分類学
  • 生態学
  • 植物科学


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