An oxidized nucleotide affects DNA replication through activation of protein kinases in Xenopus egg lysates

Toshinori Kai, Rieko Matsunaga, Masami Eguchi, Hiroyuki Kamiya, Hiroshi Kasai, Motoshi Suzuki, Shunji Izuta

研究成果: Article査読

7 被引用数 (Scopus)

抄録

To elucidate the response to oxidative stress in eukaryotic cells, the effect of an oxidized nucleotide, 8-oxo-2′-deoxyguanosine 5′-triphosphate (8-oxo-dGTP), generated from dGTP with an active oxygen, on DNA synthesis was studied using a cell-free DNA replication system derived from Xenopus egg lysates with a single-stranded DNA template. Amounts of newly synthesized DNA were reduced according to the increasing concentration of 8-oxo-dGTP. Pulse labeling analysis revealed that 8-oxo-dGTP could delay DNA synthesis by reducing the rate of chain elongation. This delay was recovered by addition of a protein kinase inhibitor, staurosporine or bisindolylmaleimide I. These results indicate that a staurosporine- or bisindolylmaleimide I-sensitive protein kinase, such as a protein kinase C family member, may contribute to the delay of DNA synthesis by 8-oxo-dGTP. UV-irradiated single-stranded DNA also caused a delay of DNA synthesis on the undamaged template in the lysates. However, this delay was not recovered by staurosporine or bisindolylmaleimide I. Therefore, the mechanism of delay of DNA synthesis by 8-oxo-dGTP may be different from that by UV lesions. This is the first report that demonstrates an effect of an oxidized nucleotide on DNA replication in eukaryotes.

本文言語English
ページ(範囲)569-573
ページ数5
ジャーナルNucleic acids research
30
2
DOI
出版ステータスPublished - 15-01-2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学

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