Analysis of canine herpesvirus gB, gC and gD expressed by a recombinant vaccinia virus

X. Xuan, A. Kojima, T. Murata, T. Mikami, H. Otsuka

研究成果: Article査読

10 被引用数 (Scopus)

抄録

The genes encoding the canine herpesvirus (CHV) glycoprotein B (gB), gC and gD homologues have been reported already. However, products of these genes have not been identified yet. Previously, we have identified three CHV glycoproteins, gp145/112, gp80 and gp47 using a panel of monoclonal antibodies (MAbs). To determine which CHV glycoprotein corresponds to gB, gC or gD, the putative genes of gB, gC, and gD of CHV were inserted into the thymidine kinase gene of vaccinia virus LC16mO strain under the control of the early-late promoter for the vaccinia virus 7.5-kilodalton polypeptide. We demonstrated here that gp145/112, gp80 and gp47 were the translation products of the CHV gB, gC and gD genes, respectively. The antigenic authenticity of recombinant gB, gC and gD were confirmed by a panel of MAbs specific for each glycoprotein produced in CHV-infected cells. Immunization of mice with these recombinants produced high titers of neutralizing antibodies against CHV. These results suggest that recombinant vaccinia viruses expressing CHV gB, gC and gD may be useful to develop a vaccine to control CHV infection.

本文言語English
ページ(範囲)1003-1010
ページ数8
ジャーナルArchives of Virology
142
5
DOI
出版ステータスPublished - 1997
外部発表はい

All Science Journal Classification (ASJC) codes

  • ウイルス学

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