TY - JOUR
T1 - Analysis of cardiomyocyte movement in the developing murine heart
AU - Hashimoto, Hisayuki
AU - Yuasa, Shinsuke
AU - Tabata, Hidenori
AU - Tohyama, Shugo
AU - Seki, Tomohisa
AU - Egashira, Toru
AU - Hayashiji, Nozomi
AU - Hattori, Fumiyuki
AU - Kusumoto, Dai
AU - Kunitomi, Akira
AU - Takei, Makoto
AU - Kashimura, Shin
AU - Yozu, Gakuto
AU - Shimojima, Masaya
AU - Motoda, Chikaaki
AU - Muraoka, Naoto
AU - Nakajima, Kazunori
AU - Sakaue-Sawano, Asako
AU - Miyawaki, Atsushi
AU - Fukuda, Keiichi
N1 - Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.
PY - 2015/9/4
Y1 - 2015/9/4
N2 - The precise assemblage of several types of cardiac precursors controls heart organogenesis. The cardiac precursors show dynamic movement during early development and then form the complicated heart structure. However, cardiomyocyte movements inside the newly organized mammalian heart remain unclear. We previously established the method of ex vivo time-lapse imaging of the murine heart to study cardiomyocyte behavior by using the Fucci (fluorescent ubiquitination-based cell cycle indicator) system, which can effectively label individual G1, S/G2/M, and G1/S-transition phase nuclei in living cardiomyocytes as red, green, and yellow, respectively. Global analysis of gene expression in Fucci green positive ventricular cardiomyocytes confirmed that cell cycle regulatory genes expressed in G1/S, S, G2/M, and M phase transitions were upregulated. Interestingly, pathway analysis revealed that many genes related to the cell cycle were significantly upregulated in the Fucci green positive ventricular cardiomyocytes, while only a small number of genes related to cell motility were upregulated. Time-lapse imaging showed that murine proliferating cardiomyocytes did not exhibit dynamic movement inside the heart, but stayed on site after entering the cell cycle.
AB - The precise assemblage of several types of cardiac precursors controls heart organogenesis. The cardiac precursors show dynamic movement during early development and then form the complicated heart structure. However, cardiomyocyte movements inside the newly organized mammalian heart remain unclear. We previously established the method of ex vivo time-lapse imaging of the murine heart to study cardiomyocyte behavior by using the Fucci (fluorescent ubiquitination-based cell cycle indicator) system, which can effectively label individual G1, S/G2/M, and G1/S-transition phase nuclei in living cardiomyocytes as red, green, and yellow, respectively. Global analysis of gene expression in Fucci green positive ventricular cardiomyocytes confirmed that cell cycle regulatory genes expressed in G1/S, S, G2/M, and M phase transitions were upregulated. Interestingly, pathway analysis revealed that many genes related to the cell cycle were significantly upregulated in the Fucci green positive ventricular cardiomyocytes, while only a small number of genes related to cell motility were upregulated. Time-lapse imaging showed that murine proliferating cardiomyocytes did not exhibit dynamic movement inside the heart, but stayed on site after entering the cell cycle.
UR - https://www.scopus.com/pages/publications/84940462302
UR - https://www.scopus.com/pages/publications/84940462302#tab=citedBy
U2 - 10.1016/j.bbrc.2015.07.036
DO - 10.1016/j.bbrc.2015.07.036
M3 - Article
C2 - 26168730
AN - SCOPUS:84940462302
SN - 0006-291X
VL - 464
SP - 1000
EP - 1007
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -