Angiogenic Role of MMP-2/9 Expressed on the Cell Surface of Early Endothelial Progenitor Cells/Myeloid Angiogenic Cells

Toshie Kanayasu-Toyoda, Takeshi Tanaka, Akiko Ishii-Watabe, Hiroko Kitagawa, Akifumi Matsuyama, Eriko Uchida, Teruhide Yamaguchi

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Since the introduction of angiogenic cell therapy using early endothelial progenitor cells (EPCs), myeloid angiogenic cells (MACs) have been expected to be useful in treating ischemic diseases. In order to elucidate the angiogenic properties of MACs/EPCs, we clarified the characteristics of MACs as compared to M2 macrophages (Mϕ{symbol}s). Comparison of the gene expression profiles of MACs and late EPCs revealed that MACs expressed greater amounts of metalloproteinase (MMP)-9. It should be noted that the profile of MMP-2/9 expression on the cell surface of MACs was similar to that of M2 Mϕ{symbol}s, and that cell surface MMP-2/9 might be an active form based on molecular size. In addition, the invasion of MACs was prohibited not only by MMP-2/9 inhibitor, but also by the hyaluronidase treatment that caused the down-regulation of MMP-9 on the cell surface of MACs and inhibited their invasion activity. These results indicate that cell surface MMP-2/9 plays an important role in the high invasion ability of MACs. The conditioned medium of both MACs and M2 Mϕ{symbol}s stimulated tube formation of endothelial cells in vitro. MACs caused an increase in vessel formation in in vivo models through the production of IL-8. We propose that the role of MACs with cell surfaces expressing MMP-2/9 is rapidly invading ischemic tissue.

本文言語English
ページ(範囲)2763-2775
ページ数13
ジャーナルJournal of Cellular Physiology
230
11
DOI
出版ステータスPublished - 01-11-2015
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生理学
  • 臨床生化学
  • 細胞生物学

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