Angiotensin II mimics the hypoxic effect on regulating trophoblast proliferation and differentiation in human placental explant cultures

Masako Araki-Taguchi, Seiji Nomura, Kazuhiko Ino, Seiji Sumigama, Eiko Yamamoto, Tomomi Kotani-Ito, Hiromi Hayakawa, Hiroaki Kajiyama, Kiyosumi Shibata, Atsuo Itakura, Fumitaka Kikkawa

研究成果: Article査読

31 被引用数 (Scopus)

抄録

Regulation of cytotrophoblast differentiation toward extravillous trophoblasts (EVTs) is critical for establishing successful pregnancy. Previous studies have focused primarily on the factors promoting the differentiation, while inhibitory regulators except hypoxia have been less documented. In this study, to test our hypothesis that angiotensin II (Ang II) would inhibit EVT differentiation, we investigated the effects of Ang II on trophoblast outgrowth and the expression of molecules associated with the proliferation and invasion of trophoblasts using human first trimester villous explant cultures. Ang II increased EVT outgrowth and the number of cells in cell columns. Moreover, Ang II-treated explants exhibited increased Ki67 and integrin α5 immunoreactivity in EVTs as well as matrix metalloproteinase-2 activity in the conditioned media, and decreased α1 integrin immunoreactivity, which are compatible with the features of the proliferative phenotype EVTs. These effects of Ang II were similar to those of hypoxia (3% O2). Ang II stimulated the expression of hypoxia inducible factor-1α at both mRNA and protein levels, and also enhanced the expression of plasminogen activator inhibitor-1 (PAI-1). Data presented herein suggest a possible role for Ang II in impairing trophoblast differentiation toward an invasive phenotype, which might be associated with shallow invasion in preeclamptic placentas.

本文言語English
ページ(範囲)59-67
ページ数9
ジャーナルLife Sciences
82
1-2
DOI
出版ステータスPublished - 02-01-2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 薬理学、毒性学および薬学(全般)

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