Two types of anion channel blockers, SITS (4‐acetamide‐4‘‐isothiocyanostilbene‐2,2′‐disulfonic acid) and DIDS (4,4′‐diisothiocyanostilbene‐2, 2’‐disulfonic acid), inhibited jelly‐induced acrosorne reaction in starfish and sea urchin. In starfish sperm, both of the blockers reversibly inhibited the formation of acrosomal process but they had no effect on either the acrosomal exocytosis or acid release from the sperm. Complete acrosome reaction occurred even in Cl−‐ and SO42−‐free artificial seawater whereas HCO3−was required for the acrosomal exocytosis. Importance of anion transport in acrosome reaction is discussed.
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