TY - JOUR
T1 - Anion Channel Blockers Inhibit the Acrosome Reaction of Echinoderm Sperm
T2 - anion channel blocker/acrosome reaction/acid release/stafish/sea urchin
AU - NISHIYAMA, ICHIRO
AU - SASAKI, HAJIME
AU - MATSUI, TAEI
AU - HOSHI, MOTONORI
PY - 1985
Y1 - 1985
N2 - Two types of anion channel blockers, SITS (4‐acetamide‐4‘‐isothiocyanostilbene‐2,2′‐disulfonic acid) and DIDS (4,4′‐diisothiocyanostilbene‐2, 2’‐disulfonic acid), inhibited jelly‐induced acrosorne reaction in starfish and sea urchin. In starfish sperm, both of the blockers reversibly inhibited the formation of acrosomal process but they had no effect on either the acrosomal exocytosis or acid release from the sperm. Complete acrosome reaction occurred even in Cl−‐ and SO42−‐free artificial seawater whereas HCO3−was required for the acrosomal exocytosis. Importance of anion transport in acrosome reaction is discussed.
AB - Two types of anion channel blockers, SITS (4‐acetamide‐4‘‐isothiocyanostilbene‐2,2′‐disulfonic acid) and DIDS (4,4′‐diisothiocyanostilbene‐2, 2’‐disulfonic acid), inhibited jelly‐induced acrosorne reaction in starfish and sea urchin. In starfish sperm, both of the blockers reversibly inhibited the formation of acrosomal process but they had no effect on either the acrosomal exocytosis or acid release from the sperm. Complete acrosome reaction occurred even in Cl−‐ and SO42−‐free artificial seawater whereas HCO3−was required for the acrosomal exocytosis. Importance of anion transport in acrosome reaction is discussed.
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U2 - 10.1111/j.1440-169X.1985.00461.x
DO - 10.1111/j.1440-169X.1985.00461.x
M3 - Article
AN - SCOPUS:0022387605
VL - 27
SP - 461
EP - 468
JO - Development Growth and Differentiation
JF - Development Growth and Differentiation
SN - 0012-1592
IS - 4
ER -