Antibiotic releasing hydroxyapatite beads

Takashi Osada, Keiko Yamamura, Kohji Yano, Toshitaka Nabeshima, Hisashi Iwata, Toshihisa Yotsuyanagi

研究成果: Article

抜粋

Synthesis hydroxyapatite (HAP), similar to bone or tooth mineral composition, was developed for bone grafts in dental and orthopedic applications. Common skin microorganisms, such as Staphylococcus aureus and Staphylococcus epidermidis, have been associated with bone graft-related infections. We investigated whether an antibiotic controlled-releasing HAP device would reduce bacterial organisms at the operated site. Cefotiam (CTM) was used as a model antibiotic since it possesses activity against Staphylococcus aureus and Staphylococcus epidermidis. To control the release rate of CTM, egg phosphatidylcholine (EPC) was loaded into a HAP bead (diameter : 8.48 mm, weight : 531 mg). The EPC-HAP beads were placed in CTM aqueous solutions(4-25 mg/ml) under atmospheric pressure. A bead containing CTM solution in its internal pores was referred to as a “wet CTM-EPC-HAP bead”. In vitro release studies demonstrated that the release rate of CTM decreased with the increase of EPC load. One wet CTM-EPC-HAP bead was implanted in the skin pocket made in a rat, and inoculated with Staphylococcus aureus or Staphylococcus epidermidis (107CFU/ml). Wet CTM-EPC-HAP beads showed less Staphylococcus aureus and Staphylococcus epidermidis in comparison with the control HAP beads two days after inoculation. These studies suggest that antibiotic controlled-releasing HAP delivery can decrease infections at the operated site of patients undergoing bone grafts.

元の言語English
ページ(範囲)431-435
ページ数5
ジャーナルDrug Delivery System
10
発行部数6
DOI
出版物ステータスPublished - 01-01-1995
外部発表Yes

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

これを引用

Osada, T., Yamamura, K., Yano, K., Nabeshima, T., Iwata, H., & Yotsuyanagi, T. (1995). Antibiotic releasing hydroxyapatite beads. Drug Delivery System, 10(6), 431-435. https://doi.org/10.2745/dds.10.431