Antibody fusions with fluorescent proteins: A versatile reagent for profiling protein expression

Kazuhiko Morino, Harue Katsumi, Yasushi Akahori, Yoshitaka Iba, Midori Shinohara, Yoshinori Ukai, Yuji Kohara, Yoshikazu Kurosawa

研究成果: Article査読

51 被引用数 (Scopus)

抄録

We developed a system by which antibodies, fused to fluorescent proteins with different wavelengths, can be prepared within a month against various antigens. An antibody library composed of a large number of single-chain Fv-CL fragment was constructed by means of a phage-display system. The constructs were designed to facilitate changing of the protein forms by simple enzyme manipulation. In the present study, we adopted a molecular form of antibody in which a single-chain Fv-CL fragment is fused with a green fluorescent protein (GFP) or red fluorescent protein (RFP). In addition, a His-tag was inserted between CL and GFP (or RFP). We describe the utility of this system using Caenorhabditis elegans embryo as a model.

本文言語English
ページ(範囲)175-184
ページ数10
ジャーナルJournal of immunological methods
257
1-2
DOI
出版ステータスPublished - 01-11-2001

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学

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