TY - JOUR
T1 - Antibody fusions with fluorescent proteins
T2 - A versatile reagent for profiling protein expression
AU - Morino, Kazuhiko
AU - Katsumi, Harue
AU - Akahori, Yasushi
AU - Iba, Yoshitaka
AU - Shinohara, Midori
AU - Ukai, Yoshinori
AU - Kohara, Yuji
AU - Kurosawa, Yoshikazu
N1 - Funding Information:
This work was supported in parts by a Grant-in-Aid for Research on Advanced Medical Technology, from the Ministry of Health and Welfare; Science and Technology Agency; and the Ministry of Education, Science and Culture of Japan.
PY - 2001/11/1
Y1 - 2001/11/1
N2 - We developed a system by which antibodies, fused to fluorescent proteins with different wavelengths, can be prepared within a month against various antigens. An antibody library composed of a large number of single-chain Fv-CL fragment was constructed by means of a phage-display system. The constructs were designed to facilitate changing of the protein forms by simple enzyme manipulation. In the present study, we adopted a molecular form of antibody in which a single-chain Fv-CL fragment is fused with a green fluorescent protein (GFP) or red fluorescent protein (RFP). In addition, a His-tag was inserted between CL and GFP (or RFP). We describe the utility of this system using Caenorhabditis elegans embryo as a model.
AB - We developed a system by which antibodies, fused to fluorescent proteins with different wavelengths, can be prepared within a month against various antigens. An antibody library composed of a large number of single-chain Fv-CL fragment was constructed by means of a phage-display system. The constructs were designed to facilitate changing of the protein forms by simple enzyme manipulation. In the present study, we adopted a molecular form of antibody in which a single-chain Fv-CL fragment is fused with a green fluorescent protein (GFP) or red fluorescent protein (RFP). In addition, a His-tag was inserted between CL and GFP (or RFP). We describe the utility of this system using Caenorhabditis elegans embryo as a model.
UR - https://www.scopus.com/pages/publications/0035514481
UR - https://www.scopus.com/pages/publications/0035514481#tab=citedBy
U2 - 10.1016/S0022-1759(01)00462-8
DO - 10.1016/S0022-1759(01)00462-8
M3 - Article
C2 - 11687251
AN - SCOPUS:0035514481
SN - 0022-1759
VL - 257
SP - 175
EP - 184
JO - Journal of immunological methods
JF - Journal of immunological methods
IS - 1-2
ER -