Application of efficient and specific gene transfer systems and organ culture techniques for the elucidation of mechanisms of epithelial-mesenchymal interaction in the developing gut

Kimiko Fukuda, Nobuyuki Sakamoto, Tomohiro Narita, Kanako Saitoh, Takashi Kameda, Hideo Iba, Sadao Yasugi

研究成果: ジャーナルへの寄稿簡易調査査読

36 被引用数 (Scopus)

抄録

Epithelial-mesenchymal interactions are very important in the development of the vertebrate gut. In the avian embryonic stomach (proventriculus), expression of embryonic chick pepsinogen (ECPg) gene, which is specific to developing glandular cells in stomach epithelium, is regulated by mesenchymal influence. Molecular mechanisms of tissue-specific transcriptional regulation of the ECPg gene and the molecular nature of the mesenchymal signals were analyzed using a combination of the classic organ culture system and gene transfer strategies. In the present review, three methods for the introduction of DNA into tissues are described: lipofection, electroporation and retroviral infection, and characteristics of each system are discussed.

本文言語英語
ページ(範囲)207-211
ページ数5
ジャーナルDevelopment Growth and Differentiation
42
3
DOI
出版ステータス出版済み - 2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • 発生生物学
  • 細胞生物学

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