Appropriate Epitope Retrieval for c-kit Protein Immunostaining in Routinely Prepared Specimens of Gastrointestinal Stromal Tumor

Atsuji Matsuyama, Shingo Kamoshida, Yoshikazu Mizoguchi, Ryoichi Shimomura, Yutaka Hirasawa, Ken Ichi Inada, Yutaka Tsutsumi

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Gastrointestinal stromal tumor (GIST), immunohistochemically characterized by c-kit protein (CD117) expression, is known to be resistent to chemotherapy and radiotherapy when it is malignant. Recently, Gleevec (STI571) has been introduced for the therapy of metastatic GISTs, and appropriate evaluation of c-kit protein has thus been clinically relevant to confirm the indication of Gleevec therapy. However, we often experienced the instability of immunohistochemical staining for c-kit protein. The aim of the present study is to establish a reproducible immunohistochemical procedure for c-kit protein detection in paraffin sections. Regarding the fixation period, formalin fixation for 24 to 72 hours gave the best result among several fixation conditions. For the purpose of epitope retrieval, hydrated heating in EDTA solution, pH 8.0, employing a pressure cooker gave the most reproducible immunostaining result. For visualization of the reaction products, diaminobenzidine solution available from DakoCytomation Co. was the most sensitive. Under these conditions, c-kit protein immunoreactivity was clearly observed in 20 (95%) of 21 GISTs, routinely processed for histologic preparation. Two tumors were proven to be false negative by the routine diagnostic procedure.

本文言語English
ページ(範囲)87-93
ページ数7
ジャーナルActa Histochemica et Cytochemica
37
2
DOI
出版ステータスPublished - 2004

All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 生化学
  • 生理学
  • 組織学
  • 細胞生物学

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