Aqueous fraction of Sauropus androgynus might be responsible for bronchiolitis obliterans

Izumi Hashimoto, Kazuyoshi Imaizumi, Naozumi Hashimoto, Hiroshi Furukawa, Yukihiro Noda, Tsutomu Kawabe, Toyohiro Honda, Tomomi Ogawa, Masaki Matsuo, Naoyuki Imai, Satoru Ito, Mitsuo Sato, Masashi Kondo, Kaoru Shimokata, Yoshinori Hasegawa

研究成果: ジャーナルへの寄稿学術論文査読

8 被引用数 (Scopus)

抄録

Background and objective: Bronchiolitis obliterans (BO) has been reported to develop following ingestion of Sauropus androgynus (SA), a leafy shrub distributed in Southeast Asia. Little is known about direct effects of SA on airway resident cells or haematopoietic cells in vitro. Identification of the SA component responsible for the development of BO would be an important key to elucidate its mechanism. We sought to elucidate the direct effects of SA on airway resident cells or haematopoietic cells and identify the SA element responsible for the pathogenesis of BO. Methods: SA dry powder was partitioned into fractions by solvent extraction. Human and murine monocytic cells, epithelial cells and endothelial cells were cultured with SA solution or fractions eluted from SA. We also investigated the effect of SA in vivo using a murine BO syndrome (BOS) model. Results: The aqueous fraction of SA induced significant increases of inflammatory cytokine and chemokine production from monocytic lineage cells. This fraction also induced significant apoptosis of endothelial cells and enhanced intraluminal obstructive fibrosis in allogeneic trachea allograft in the murine BOS model. We found individual differences in tumour necrosis factor α (TNF-α) production from monocytes of healthy controls stimulated by this aqueous fraction of SA, whereas it induced high-level TNF-α production from monocytes of patients with SA-induced BO. Conclusions: These results suggest that an aqueous fraction of SA may be responsible for the pathogenesis of BO. Ingestion of Sauropus androgynus (SA) as dry powder or fresh juice has been reported to induce constrictive bronchiolitis obliterans (BO), but the pathogenesis of this condition is unknown. This study suggests that an aqueous fraction of SA could be responsible for development of SA-induced BO.

本文言語英語
ページ(範囲)340-347
ページ数8
ジャーナルRespirology
18
2
DOI
出版ステータス出版済み - 02-2013

All Science Journal Classification (ASJC) codes

  • 呼吸器内科

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