Arachidonic acid-induced Ca2+ sensitization of smooth muscle contraction through activation of Rho-kinase

Shigemasa Araki, Masaaki Ito, Yasuko Kureishi, Jianhua Feng, Hirofumi Machida, Naoki Isaka, Mutsuki Amano, Kozo Kaibuchi, David J. Hartshorne, Takeshi Nakano

研究成果: Article

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Arachidonic acid activates isolated Rho-kinase and contracts permeabilized smooth muscle fibres. Various assays were carried out to examine the mechanism of this activation. Native Rho-kinase was activated 5-6 times by arachidonic acid but an N-terminal, constitutively-active fragment of Rho-kinase, expressed as a glutathione-S-transferase (GST) fusion protein and including the catalytic subunit (GST-Rho-kinase-CAT), was not. GST-Rho-kinase-CAT was inhibited by a C-terminal fragment of Rho-kinase and arachidonic acid removed this inhibition. These results suggest that the C-terminal part of Rho-kinase, containing the RhoA binding site and the pleckstrin homology domain, acts as an autoinhibitor. It is suggested further that activation by arachidonic acid is due to its binding to the autoinhibitory region and subsequent release from the catalytic site. Arachidonic acid, at concentrations greater than 30 μM, increases force in α-toxin-permeabilized femoral artery but not in Triton X-100-skinned fibres. The content of Rho-kinase in the latter was lower than in α-toxin-treated or intact fibres. The arachidonic acid-induced contraction was not observed at a pCa above 8.0 and was inhibited by Y-27632 and wortmannin, inhibitors of Rho-kinase and myosin light-chain kinase (MLCK), respectively. The activation of Rho-kinase and subsequent phosphorylation of the myosin phosphatase target sub-unit inhibits myosin phosphatase and increases myosin phosphorylation.

ジャーナルPflugers Archiv European Journal of Physiology
出版物ステータスPublished - 12-03-2001

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

フィンガープリント Arachidonic acid-induced Ca<sup>2+</sup> sensitization of smooth muscle contraction through activation of Rho-kinase' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Araki, S., Ito, M., Kureishi, Y., Feng, J., Machida, H., Isaka, N., Amano, M., Kaibuchi, K., Hartshorne, D. J., & Nakano, T. (2001). Arachidonic acid-induced Ca2+ sensitization of smooth muscle contraction through activation of Rho-kinase. Pflugers Archiv European Journal of Physiology, 441(5), 596-603.