Arsenic trioxide induces apoptosis in neuroblastoma cell lines through the activation of caspase 3 in vitro

Yukihiro Akao, Yoshihito Nakagawa, Kiyotaka Akiyama

研究成果: Article査読

181 被引用数 (Scopus)

抄録

Arsenic trioxide (As2O3) induces clinical remission in acute promyelocytic leukemia, even in all-trans retinoic acid-refractory cases, with minimal toxicity at low (1-2 μM) concentration. We exposed various neuroblastoma cell lines to As2O3 at a concentration of 2 μM: as a result, seven of 10 neuroblastoma cell lines underwent apoptosis characterized by morphological changes and nucleosomal DNA fragmentation. As2O3-induced apoptosis in neuroblastoma cells was shown to occur through the activation of caspase 3, as judged from Western blot analysis and apoptosis inhibition assay. It seemed that the sensitivity of neuroblastoma cells to As2O3 was inversely proportional to their intracellular level of reduced glutathione. Taken together these results indicate that As2O3 would be a candidate as a therapeutic agent for treatment of neuroblastoma, which is a solid tumor, not only by systemic therapy but also by local therapy. Copyright (C) 1999 Federation of European Biochemical Societies.

本文言語English
ページ(範囲)59-62
ページ数4
ジャーナルFEBS Letters
455
1-2
DOI
出版ステータスPublished - 16-07-1999
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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