Association between brain and plasma glutamine levels in healthy young subjects investigated by MRS and LC/MS

Yuhei Takado, Naoto Sato, Yuta Kanbe, Moyoko Tomiyasu, Lijing Xin, Jamie Near, Kohki Yoshikawa, Naruhiko Sahara, Tatsuya Higashi, Tetsuya Suhara, Makoto Higuchi, Takayuki Obata

研究成果: ジャーナルへの寄稿学術論文査読

20 被引用数 (Scopus)

抄録

Both glutamine (Gln) and glutamate (Glu) are known to exist in plasma and brain. However, despite the assumed relationship between brain and plasma, no studies have clarified the association betweenthem. Proton magnetic resonance spectroscopy (MRS) was sequentially performed twice, with a 60-min interval, on 10 males and 10 females using a 3T scanner. Blood samples for liquid chromatography-mass spectrometry (LC/MS) to measure Gln and Glu concentrations in plasma were collected during the time interval between the two MRS sessions.MRSvoxels of interest were localized at the posterior cingulate cortex (PCC) and cerebellum (Cbll) and measured by the SPECIAL sequence. Spearman’s correlation coefficient was used to examine t e associationbetween brain and plasma metabolites. The Gln concentrations in PCC (mean of two measurements) were positively correlated with Gln concentrations in plasma (p < 0.01, r = 0.72). However, the Glu concentrations in the two regions were not correlated with those in plasma. Cons deration of the different dynamics of Gln and Glu between plasma and brain is crucial when addressing the pathomechanism and therapeutic strategies for brain disorders such as Alzheimer’s disease and hepatic encephalopathy.

本文言語英語
論文番号1649
ジャーナルNutrients
11
7
DOI
出版ステータス出版済み - 07-2019
外部発表はい

All Science Journal Classification (ASJC) codes

  • 食品科学
  • 栄養および糖尿病

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