Association of a novel long non-coding RNA in 8q24 with prostate cancer susceptibility

Suyoun Chung, Hidewaki Nakagawa, Motohide Uemura, Lianhua Piao, Kyota Ashikawa, Naoya Hosono, Ryo Takata, Shusuke Akamatsu, Takahisa Kawaguchi, Takashi Morizono, Tatsuhiko Tsunoda, Yataro Daigo, Koichi Matsuda, Naoyuki Kamatani, Yusuke Nakamura, Michiaki Kubo

研究成果: Article査読

244 被引用数 (Scopus)

抄録

Recent genome-wide association studies reported strong and reproducible associations of multiple genetic variants in a large "gene-desert" region of chromosome 8q24 with susceptibility to prostate cancer (PC). However, the causative or functional variants of these 8q24 loci and their biological mechanisms associated with PC susceptibility remain unclear and should be investigated. Here, focusing on its most centromeric region (so-called Region 2: Chr8: 128.14-128.28 Mb) among the multiple PC loci on 8q24, we performed fine mapping and re-sequencing of this critical region and identified SNPs (single nucleotide polymorphisms) between rs1456315 and rs7463708 (chr8: 128,173,119-128,173,237 bp) to be most significantly associated with PC susceptibility (P = 2.00 × 10-24, OR = 1.74, 95% confidence interval = 1.56-1.93). Importantly, we show that this region was transcribed as a 13 kb intron-less long non-coding RNA (ncRNA), termed PRNCR1 (prostate cancer non-coding RNA 1), and PRNCR1 expression was upregulated in some of the PC cells as well as precursor lesion prostatic intraepithelial neoplasia. Knockdown of PRNCR1 by siRNA attenuated the viability of PC cells and the transactivation activity of androgen receptor, which indicates that PRNCR1 could be involved in prostate carcinogenesis possibly through androgen receptor activity. These findings could provide a new insight in understanding the pathogenesis of genetic factors for PC susceptibility and prostate carcinogenesis.

本文言語English
ページ(範囲)245-252
ページ数8
ジャーナルCancer science
102
1
DOI
出版ステータスPublished - 01-2011
外部発表はい

All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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