TY - JOUR
T1 - Association of smoking habits with TXNIP DNA methylation levels in leukocytes among general Japanese population
AU - Maeda, Keisuke
AU - Yamada, Hiroya
AU - Munetsuna, Eiji
AU - Fujii, Ryosuke
AU - Yamazaki, Mirai
AU - Ando, Yoshitaka
AU - Mizuno, Genki
AU - Ishikawa, Hiroaki
AU - Ohashi, Koji
AU - Tsuboi, Yoshiki
AU - Hashimoto, Shuji
AU - Hamajima, Nobuyuki
AU - Suzuki, Koji
N1 - Publisher Copyright:
© 2020 Maeda et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2020/7
Y1 - 2020/7
N2 - Thioredoxin-interacting protein (TXNIP) inhibits the activity of thioredoxin (TXN), leading to increased oxidative stress. Expression of the TXNIP gene is regulated by DNA methylation. However, no study has reported the influence of lifestyle factors on TXNIP DNA methylation. Our goal was to determine the association between smoking habits and TXNIP DNA methylation levels in a Japanese population. We conducted a cross-sectional study of 417 subjects (180 males and 237 females) participating in a health examination. We used a pyrosequencing assay to determine TXNIP DNA methylation levels in leukocytes. The mean TXNIP DNA methylation level in current smokers (75.3%) was significantly lower than that in never and ex-smokers (never: 78.1%, p < 0.001; ex: 76.9%, p = 0.013). Multivariable logistic regression analyses showed that the OR for TXNIP DNA hypomethylation was significantly higher in current smokers than that in never smokers, and significantly higher in current smokers with years of smoking ≥ 35 and Brinkman Index ≥ 600 compared to that in nonsmokers. In conclusion, we found that current smokers had TXNIP DNA hypomethylation compared to never and ex-smokers. Moreover, long-term smoking and high smoking exposure also were associated with TXNIP DNA hypomethylation.
AB - Thioredoxin-interacting protein (TXNIP) inhibits the activity of thioredoxin (TXN), leading to increased oxidative stress. Expression of the TXNIP gene is regulated by DNA methylation. However, no study has reported the influence of lifestyle factors on TXNIP DNA methylation. Our goal was to determine the association between smoking habits and TXNIP DNA methylation levels in a Japanese population. We conducted a cross-sectional study of 417 subjects (180 males and 237 females) participating in a health examination. We used a pyrosequencing assay to determine TXNIP DNA methylation levels in leukocytes. The mean TXNIP DNA methylation level in current smokers (75.3%) was significantly lower than that in never and ex-smokers (never: 78.1%, p < 0.001; ex: 76.9%, p = 0.013). Multivariable logistic regression analyses showed that the OR for TXNIP DNA hypomethylation was significantly higher in current smokers than that in never smokers, and significantly higher in current smokers with years of smoking ≥ 35 and Brinkman Index ≥ 600 compared to that in nonsmokers. In conclusion, we found that current smokers had TXNIP DNA hypomethylation compared to never and ex-smokers. Moreover, long-term smoking and high smoking exposure also were associated with TXNIP DNA hypomethylation.
UR - http://www.scopus.com/inward/record.url?scp=85087470695&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85087470695&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0235486
DO - 10.1371/journal.pone.0235486
M3 - Article
C2 - 32609762
AN - SCOPUS:85087470695
SN - 1932-6203
VL - 15
JO - PloS one
JF - PloS one
IS - 7
M1 - e0235486
ER -