Attenuation of fibroblast growth factor signaling by poly-N- acetyllactosamine type glycans

Kazuhiro Sugihara, Toshiaki K. Shibata, Kayoko Takata, Takako Kimura, Naohiro Kanayama, Roy Williams, Shingo Hatakeyama, Tomoya O. Akama, Chu Wei Kuo, Kay Hooi Khoo, Michiko N. Fukuda

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glycans (polyLacs), enhanced motility. Mass spectrometry analysis revealed that sperm-associated polyLacs are heavily fucosylated, consistent with Lewis Y antigen. Immunohistochemistry of epididymis using an anti-Lewis Y antibody before and after EBG treatment suggested that polyLacs carrying the Lewis Y epitope are synthesized in epididymal epithelia and secreted to seminal fluid. EBG-treated sperm elevated cAMP levels and calcium influx, indicating activation of fibroblast growth factor signaling. Seminal fluid polyLacs bound to FGFs in vitro, and impaired FGF-mediated signaling in HEK293T cells.

本文言語English
ページ(範囲)3195-3201
ページ数7
ジャーナルFEBS Letters
587
19
DOI
出版ステータスPublished - 01-10-2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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