Autoantibody against peroxiredoxin I, an antioxidant enzyme, in patients with systemic sclerosis: Possible association with oxidative stress

Y. Iwata, F. Ogawa, K. Komura, E. Muroi, T. Hara, K. Shimizu, M. Hasegawa, M. Fujimoto, Y. Tomita, S. Sato

研究成果: Article査読

33 被引用数 (Scopus)

抄録

Objectives: To determine the prevalence and clinical correlation of autoantibody to peroxiredoxin (Prx) I, an antioxidant enzyme, in patients with systemic sclerosis (SSc). Methods: Serum samples from SSc patients (n=70) and healthy controls (n=23) were examined by ELISA using human recombinant Prx I. The presence of anti-Prx I antibody was further evaluated by immunoblotting analysis. To determine the functional relevance of anti-Prx I antibody in vivo, we assessed whether anti-Prx I antibody was able to inhibit Prx I enzymatic activity using yeast thioredoxin reductase system. Results: IgG anti-Prx I antibody levels in SSc patients were significantly higher than healthy controls and this autoantibody was detected in 33% of SSc patients. The presence of IgG anti-Prx I antibody was associated with longer disease duration, more frequent presence of pulmonary fibrosis, heart involvement, and anti-topoisomerase I antibody and increased levels of serum immunoglobulin and erythrocyte sedimentation rates. IgG anti-Prx I antibody levels also correlated positively with renal vascular damage and negatively with pulmonary function tests. Furthermore, anti-Prx I antibody levels correlated positively with serum levels of 8-isoprostane, a marker of oxidative stress. Immunoblotting analysis confirmed the presence of anti-Prx I antibody. Remarkably, Prx I enzymatic activity was inhibited by IgG isolated from SSc sera containing IgG anti-Prx I antibody. Conclusions: These results suggest that elevated IgG anti-Prx I autoantibody is associated with the disease severity of SSc and that anti-PrxI antibody may enhance the oxidative stress by inhibiting Prx I enzymatic activity.

本文言語English
ページ(範囲)790-795
ページ数6
ジャーナルRheumatology
46
5
DOI
出版ステータスPublished - 05-2007
外部発表はい

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Pharmacology (medical)

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