TY - JOUR
T1 - Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells
AU - Islam, Shamima
AU - Hassan, Ferdaus
AU - Tumurkhuu, Gantsetseg
AU - Dagvadorj, Jargalsaikhan
AU - Koide, Naoki
AU - Naiki, Yoshikazu
AU - Mori, Isamu
AU - Yoshida, Tomoaki
AU - Yokochi, Takashi
N1 - Funding Information:
This work was supported by in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan. We are grateful to K. Takahashi and A. Morikawa for the technical assistance.
PY - 2007/8/24
Y1 - 2007/8/24
N2 - Lipopolysaccharide (LPS) is a potent bone resorbing factor. The effect of LPS on osteoclast formation was examined by using murine RAW 264.7 macrophage cells. LPS-induced the formation of multinucleated giant cells (MGC) in RAW 264.7 cells 3 days after the exposure. MGCs were positive for tartrate-resistant acid phosphatase (TRAP) activity. Further, MGC formed resorption pits on calcium-phosphate thin film that is a substrate for osteoclasts. Therefore, LPS was suggested to induce osteoclast formation in RAW 264.7 cells. LPS-induced osteoclast formation was abolished by anti-tumor necrosis factor (TNF)-α antibody, but not antibodies to macrophage-colony stimulating factor (M-CSF) and receptor activator of nuclear factor (NF)-κB ligand (RANKL). TNF-α might play a critical role in LPS-induced osteoclast formation in RAW 264.7 cells. Inhibitors of NF-κB and stress activated protein kinase (SAPK/JNK) prevented the LPS-induced osteoclast formation. The detailed mechanism of LPS-induced osteoclast formation is discussed.
AB - Lipopolysaccharide (LPS) is a potent bone resorbing factor. The effect of LPS on osteoclast formation was examined by using murine RAW 264.7 macrophage cells. LPS-induced the formation of multinucleated giant cells (MGC) in RAW 264.7 cells 3 days after the exposure. MGCs were positive for tartrate-resistant acid phosphatase (TRAP) activity. Further, MGC formed resorption pits on calcium-phosphate thin film that is a substrate for osteoclasts. Therefore, LPS was suggested to induce osteoclast formation in RAW 264.7 cells. LPS-induced osteoclast formation was abolished by anti-tumor necrosis factor (TNF)-α antibody, but not antibodies to macrophage-colony stimulating factor (M-CSF) and receptor activator of nuclear factor (NF)-κB ligand (RANKL). TNF-α might play a critical role in LPS-induced osteoclast formation in RAW 264.7 cells. Inhibitors of NF-κB and stress activated protein kinase (SAPK/JNK) prevented the LPS-induced osteoclast formation. The detailed mechanism of LPS-induced osteoclast formation is discussed.
KW - Jun-N-terminal kinase 1/2 (JNK1/2)
KW - Lipopolysaccharide
KW - Macrophage-colony stimulating factor
KW - Osteoclast
KW - Receptor activator of nuclear factor-κB ligand (RANKL)
KW - TNF-α
KW - Tartrate-resistant acid phosphatase (TRAP)
UR - https://www.scopus.com/pages/publications/34447109965
UR - https://www.scopus.com/pages/publications/34447109965#tab=citedBy
U2 - 10.1016/j.bbrc.2007.06.023
DO - 10.1016/j.bbrc.2007.06.023
M3 - Article
C2 - 17597583
AN - SCOPUS:34447109965
SN - 0006-291X
VL - 360
SP - 346
EP - 351
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -