Basic procedures for detection and cytotoxicity of chimeric antigen receptors

Keichiro Mihara, Tetsumi Yoshida, Joyeeta Bhattacharyya

研究成果: Chapter

1 被引用数 (Scopus)

抄録

Chimeric antigen receptors against CD19 (anti-CD19-CAR) are widely recognized and used by not only researchers associated with immunology, molecular biology, and cell biology but also physicians to treat B-cell malignancies. Anti-CD19-CAR is currently clinically available as one of the therapeutic modalities for refractory acute B-cell-typed lymphoblastic leukemia (B-ALL) patients. However, to detect CAR on the cell surface and investigate the efficacy of CAR-T cells, there are numerous experimental modalities including flow cytometry, the Cr-releasing assay, immunoblot, and immunostaining. We have chosen several techniques, which are necessary and sufficient as well as reliable and reproducible to detect and assess the killing effect of CAR-T cells. Here, we describe protocols for basic experiments and procedures for the detection of CAR on transduced cells and in in vitro coculture experiments to assess cytotoxicity using CAR-T cells.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ299-306
ページ数8
DOI
出版ステータスPublished - 01-01-2019
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
1904
ISSN(印刷版)1064-3745

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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