Binding of APC and dishevelled mediates Wnt5a-regulated focal adhesion dynamics in migrating cells

Shinji Matsumoto, Katsumi Fumoto, Tetsuji Okamoto, Kozo Kaibuchi, Akira Kikuchi

研究成果: Article査読

80 被引用数 (Scopus)

抄録

Wnt5a is a representative ligand that activates the Wnt/Β-catenin- independent pathway, resulting in the regulation of cell adhesion, migration, and polarity, but its molecular mechanism is poorly understood. This report shows that Dishevelled (Dvl) binds to adenomatous polyposis coli (APC) gene product, and this binding is enhanced by Wnt5a. Dvl was involved in the stabilization of the plus end dynamics of microtubules as well as APC. Frizzled2 (Fz2) was present with Wnt5a at the leading edge of migrating cells and formed a complex with APC through Dvl. Fz2 also interacted with integrins at the leading edge, and Dvl and APC associated with and activated focal adhesion kinase and paxillin. The binding of APC to Dvl enhanced the localization of paxillin to the leading edge and was involved in Wnt5a-dependent focal adhesion turnover. Furthermore, this new Wnt5a signalling pathway was important for the epithelial morphogenesis in the three-dimensional culture. These results suggest that the functional and physical interaction of Dvl and APC is involved in Wnt5a/Fz2-dependent focal adhesion dynamics during cell migration and epithelial morphogenesis.

本文言語English
ページ(範囲)1192-1204
ページ数13
ジャーナルEMBO Journal
29
7
DOI
出版ステータスPublished - 04-2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • 神経科学(全般)
  • 分子生物学
  • 生化学、遺伝学、分子生物学(全般)
  • 免疫学および微生物学(全般)

フィンガープリント

「Binding of APC and dishevelled mediates Wnt5a-regulated focal adhesion dynamics in migrating cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル