Biochemical and crystallographic characterization of a Rho effector domain of the protein serine/threonine kinase N in a complex with RhoA

Ryoko Maesaki, Toshiyuki Shimizu, Kentaro Ihara, Shinya Kuroda, Kozo Kaibuchi, Toshio Hakoshima

研究成果: Article査読

7 被引用数 (Scopus)

抄録

The effector domain of human protein serine/threonine kinase N (PKN), an effector protein for the small GTP-binding protein Rho, was expressed and purified for protein characterization and crystallization, in a complex form with human RhoA. In solution, RhoA binds to the PKN effector domain with 1:2 stoichiometry in a GTP-dependent manner. The obtained complex crystals diffract to 2.2 Å resolution.

本文言語English
ページ(範囲)166-170
ページ数5
ジャーナルJournal of Structural Biology
126
2
DOI
出版ステータスPublished - 15-06-1999
外部発表はい

All Science Journal Classification (ASJC) codes

  • 構造生物学

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