Bioluminescent bacteria have potential as a marker of drowning in seawater: Two immersed cadavers retrieved near estuaries

Eiji Kakizaki, Shuji Kozawa, Masahiro Sakai, Nobuhiro Yukawa

研究成果: Article査読

23 被引用数 (Scopus)

抄録

We detected numerous bioluminescent bacteria in blood samples from two cadavers that had been immersed in estuarine environments. Autopsy, diatomaceous and toxicological findings indicated death by drowning, which agreed with environmental aspects and the findings of police investigations. Bioluminescent bacteria appeared in blood samples cultured on selective agar containing 2%, 3% and 4% NaCl after about 18 h. Blood from the left side of the heart, the right side of the heart and the femoral vein generated 7.0 × 102, 2.0 × 104 and 8.0 × 102 cfu/ml of blood (case 1), and 1.8 × 104, 1.1 × 103 and 2.5 × 101 cfu/ml (case 2) of bioluminescent colonies, respectively, in agar containing 4% NaCl. Homologous analysis based on the 16S rRNA gene also identified the bioluminescent colonies as Vibrio fischeri and V. harveyi, which normally inhabit seawater. This simple assay might serve as an additional indicator to support a conclusion of death by drowning together with the diatom test.

本文言語English
ページ(範囲)91-96
ページ数6
ジャーナルLegal Medicine
11
2
DOI
出版ステータスPublished - 03-2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 諸問題の倫理的、法的側面

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