Biosynthesis of the so-called “a” and “asialo” pathway glycosphingolipids is differentially regulated in murine myelogenous leukemia nfs60 cells

Mitsuru Nakamura, Takao Sakai, Yusuke Furukawa, Seiichi Kitagawa, Kumi Sakoe, Atsuko Tsunoda

研究成果: ジャーナルへの寄稿学術論文査読

1 被引用数 (Scopus)

抄録

Regulation of “a” and “asialo” series ganglioside biosynthesis was analyzed. COS-1 cells expressing only GD1a showed high synthetic activities of GM3, GM2, GM1a, and GD1a, but little activity for GA2 synthesis. However, IL-3-dependent murine NFS60-I7, which has GM1b and GD1α, exhibited high synthetic activities of GM2, GM1a, and GD1a, but GM3 synthase was only 1/6 of COS-1 and GA2 synthetic activity was low. By contrast, IL-3 gene-transfected subline NFS60-H7 expressing GD1a in addition to GM1b and GD1α displayed up-regulated GM3 synthase and GA2 synthase activities, while GM2, GM1a, and GD1a synthase activities were in the same levels as in NFS60-I7 cells. Since GA2 synthetic activities were not parallel with GM2 synthase in the investigated machinery of ganglioside biosynthesis, it is strongly suggested that biosynthesis of and “asialo” series gangliosides is regulated differentially from each other in murine NFS60 cell lines.

本文言語英語
ページ(範囲)733-740
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
217
3
DOI
出版ステータス出版済み - 26-12-1995
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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