BMI-1 expression is enhanced through transcriptional and posttranscriptional regulation during the progression of chronic myeloid leukemia

Joyeeta Bhattacharyya, Keichiro Mihara, Shin'ichiro Yasunaga, Hideo Tanaka, Masaharu Hoshi, Yoshihiro Takihara, Akiro Kimura

研究成果: Article査読

36 被引用数 (Scopus)

抄録

BMI-1 plays a critical role in regulating the activity of hematopoietic stem and progenitor cells. Patients with chronic myeloid leukemia (CML) are at a risk of developing blastic crisis (BC) even after the emergence of imatinib mesylate. In this study, to determine the relevance of BMI-1 to BC, we investigated the expression of BMI-1 in CD34+ cells at each of the chronic phase (CP), the accelerated phase (AP), and BC by flow cytometry. Interestingly, the level of BMI-1 expression was significantly higher in CP than in controls and was further increased during the course of the disease progression (control - 5.66%; CP - 36.93%; AP and BC - 76.41%). Curiously, mRNA levels for BMI-1 were almost consistent during the disease progression from CP to BC (control - 2.21; CP - 9.77; AP and BC - 9.70 (BMI-1/glyceraldehyde-3-phosphate dehydrogenase ratio)). Since we further found that overexpression of BCR - ABL in human embryonic kidney-293 cells enhanced BMI-1 expression and that BMI-1 expression was increased in K562 cells, derived from patients with BC, in the presence of proteasomal inhibitors, BMI-1 was presumed to be positively regulated by BCR - ABL and further by posttranscriptional modification in the course of the disease progression. We suggest the usefulness of BMI-1 expression in CD34+ cells as a molecular marker for monitoring patients with CML.

本文言語English
ページ(範囲)333-340
ページ数8
ジャーナルAnnals of Hematology
88
4
DOI
出版ステータスPublished - 2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • Hematology

フィンガープリント 「BMI-1 expression is enhanced through transcriptional and posttranscriptional regulation during the progression of chronic myeloid leukemia」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル