Carbohydrate analysis of human von Willebrand factor with horseradish peroxidase-conjugated lectins

Taei Matsui, Chisato Kihara, Yoshihiro Fujimura, Tsuguo Mizuochi, Koiti Titani

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Human von Willebrand factor (vWF) immobilized on a polyvinylidene difluoride membrane was subjected to binding assay with a series of horseradish peroxidase-conjugated lectins. The protein was reactive with concanavalin A, Ricinus communis agglutinin 120, wheat germ agglutinin and Ulex europaeus agglutinin I (UEA-I) but not with peanut agglutinin before sialidase treatment. These reactivities were consistent with the major oligosaccharide structure reported except for UEA-I. The reactivity with UEA-I was greatly decreased after digestion of the protein with either α-L-fucosidase or peptide-N-glycosidase F, but no significant decrease was observed after mild alkaline treatment or delipidation. vWF and UEA-I have been independently used as a good marker for human endothelial cells. Our results indicate that vWF itself contains UEA-I reactive sugar chains in its Asn-linked oligosaccharides.

本文言語English
ページ(範囲)1253-1259
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
178
3
DOI
出版ステータスPublished - 15-08-1991

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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