Cardiomyoblast-like cells differentiated from human adipose tissue-derived mesenchymal stem cells improve left ventricular dysfunction and survival in a rat myocardial infarction model

Hanayuki Okura, Akifumi Matsuyama, Chun Man Lee, Ayami Saga, Aya Kakuta-Yamamoto, Anna Nagao, Nagako Sougawa, Naosumi Sekiya, Kazuhiro Takekita, Yashuhiro Shudo, Shigeru Miyagawa, Hiroshi Komoda, Teruo Okano, Yoshiki Sawa

研究成果: Article査読

47 被引用数 (Scopus)

抄録

Adipose tissue-derived mesenchymal stem cells (ADMSCs) are multipotent cells. Here we examined whether human ADMSCs (hADMSCs) could differentiate into cardiomyoblast-like cells (CLCs) by induction with dimethylsulfoxide and whether the cells would be utilized to treat cardiac dysfunction. Dimethylsulfoxide induced the expression of various cardiac markers in hADMSCs, such as α-cardiac actin, cardiac myosin light chain, and myosin heavy chain; none of which were detected in noncommitted hADMSCs. The induced cells were thus designated as hADMSC-derived CLCs (hCLCs). To confirm their beneficial effect on cardiac function, hCLC patches were transplanted onto the Nude rat myocardial infarction model, and compared with noncommitted hADMSC patch transplants and sham operations. Echocardiography demonstrated significant short-term improvement of cardiac function in both the patch-transplanted groups. However, long-term follow-up showed rescue and maintenance of cardiac function in the hCLC patch-transplanted group only, but not in the noncommitted hADMSC patch-transplanted animals. The hCLCs, but not the hADMSCs, engrafted into the scarred myocardium and differentiated into human cardiac troponin I-positive cells, and thus regarded as cardiomyocytes. Transplantation of the hCLC patches also resulted in recovery of cardiac function and improvement of long-term survival rate. Thus, transplantation of hCLC patches is a potentially effective therapeutic strategy for future cardiac tissue regeneration.

本文言語English
ページ(範囲)417-425
ページ数9
ジャーナルTissue Engineering - Part C: Methods
16
3
DOI
出版ステータスPublished - 01-06-2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオエンジニアリング
  • 医学(その他)
  • 生体医工学

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