cDNA cloning and expression of human activin βE subunit

Osamu Hashimoto, Kunihiro Tsuchida, Yuuki Ushiro, Yuko Hosoi, Nobuhiko Hoshi, Hiromu Sugino, Yoshihisa Hasegawa

研究成果: Article

37 引用 (Scopus)

抜粋

We cloned human activin βE subunit cDNA from a liver cDNA library using the polymerase chain reaction (PCR) technique. The deduced amino acid sequence was 97 and 96% homologous to the mouse and rat activin βE subunits. Human activin βE subunit tagged with Myc and polyhistidine residues at the COOH terminus was expressed in mammalian cells and secreted into the medium as a disulphide-linked homodimer protein. We also found that the human activin βE protein could bind to follistatin, an activin-binding protein. Northern blot analysis showed that this gene was expressed as a major transcript of 2.7 kb predominantly in human liver. These findings suggest that activin E (dimeric protein) may play a role in humans.

元の言語English
ページ(範囲)117-122
ページ数6
ジャーナルMolecular and Cellular Endocrinology
194
発行部数1-2
DOI
出版物ステータスPublished - 30-08-2002
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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  • これを引用

    Hashimoto, O., Tsuchida, K., Ushiro, Y., Hosoi, Y., Hoshi, N., Sugino, H., & Hasegawa, Y. (2002). cDNA cloning and expression of human activin βE subunit. Molecular and Cellular Endocrinology, 194(1-2), 117-122. https://doi.org/10.1016/S0303-7207(02)00157-0