抄録
We cloned human activin βE subunit cDNA from a liver cDNA library using the polymerase chain reaction (PCR) technique. The deduced amino acid sequence was 97 and 96% homologous to the mouse and rat activin βE subunits. Human activin βE subunit tagged with Myc and polyhistidine residues at the COOH terminus was expressed in mammalian cells and secreted into the medium as a disulphide-linked homodimer protein. We also found that the human activin βE protein could bind to follistatin, an activin-binding protein. Northern blot analysis showed that this gene was expressed as a major transcript of 2.7 kb predominantly in human liver. These findings suggest that activin E (dimeric protein) may play a role in humans.
本文言語 | 英語 |
---|---|
ページ(範囲) | 117-122 |
ページ数 | 6 |
ジャーナル | Molecular and Cellular Endocrinology |
巻 | 194 |
号 | 1-2 |
DOI | |
出版ステータス | 出版済み - 30-08-2002 |
外部発表 | はい |
All Science Journal Classification (ASJC) codes
- 生化学
- 分子生物学
- 内分泌学