cDNA cloning and expression of human activin βE subunit

Osamu Hashimoto, Kunihiro Tsuchida, Yuuki Ushiro, Yuko Hosoi, Nobuhiko Hoshi, Hiromu Sugino, Yoshihisa Hasegawa

研究成果: ジャーナルへの寄稿学術論文査読

47 被引用数 (Scopus)

抄録

We cloned human activin βE subunit cDNA from a liver cDNA library using the polymerase chain reaction (PCR) technique. The deduced amino acid sequence was 97 and 96% homologous to the mouse and rat activin βE subunits. Human activin βE subunit tagged with Myc and polyhistidine residues at the COOH terminus was expressed in mammalian cells and secreted into the medium as a disulphide-linked homodimer protein. We also found that the human activin βE protein could bind to follistatin, an activin-binding protein. Northern blot analysis showed that this gene was expressed as a major transcript of 2.7 kb predominantly in human liver. These findings suggest that activin E (dimeric protein) may play a role in humans.

本文言語英語
ページ(範囲)117-122
ページ数6
ジャーナルMolecular and Cellular Endocrinology
194
1-2
DOI
出版ステータス出版済み - 30-08-2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 内分泌学

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