C/EBPb isoforms sequentially regulate regenerating mouse hematopoietic stem/progenitor cells

Atsushi Sato, Naoka Kamio, Asumi Yokota, Yoshihiro Hayashi, Akihiro Tamura, Yasuo Miura, Taira Maekawa, Hideyo Hirai

研究成果: Article査読

抄録

The transcription factor CCAAT enhancer-binding protein b (C/EBPb) is required for stress-induced granulopoiesis at the level of hematopoietic stem/progenitor cells (HSPCs); however, its role and mechanisms of action in HSPCs are unknown. In this study, we assessed the regulation and functions of C/EBPb in HSPCs, especially under stress conditions. After 5-fluorouracil treatment or bone marrow transplantation, Cebpb2/2 HSPCs exhibited impaired cell-cycle activation and myeloid differentiation at the early and late phases of regeneration, respectively, whereas at steady state, Cebpb deficiency did not affect HSPCs. C/EBPb was upregulated in response to hematopoietic stress, especially in CD150high long term-hematopoietic stem cells (LT-HSCs). Intracellular flow cytometric analysis that detected distinct domains of C/EBPb revealed that, among the 3 isoforms of C/EBPb, liver-enriched inhibitory protein (LIP) was upregulated in LT-HSCs prior to liver-enriched activating protein (LAP)/LAP* during regeneration. Early upregulation of LIP promoted cell-cycle entry of LT-HSCs by positively regulating Myc and expanded the HSPCs pool. Subsequent myeloid differentiation of amplified HSPCs was mediated by LAP/LAP*, which were upregulated at a later phase of regeneration. Collectively, our findings show that stress-induced sequential upregulation of C/EBPb isoforms is critical for fine-tuning the proliferation and differentiation of regenerating HSPCs.

本文言語English
ページ(範囲)43-3356
ページ数3314
ジャーナルBlood Advances
32
2
DOI
出版ステータスPublished - 28-07-2020

All Science Journal Classification (ASJC) codes

  • Hematology

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