Ceramide accumulation is independent of camptothecin-induced apoptosis in prostate cancer LNCaP cells

Yukihiro Akao, Suzuno Kusakabe, Yoshiko Banno, Mariko Kito, Yoshihito Nakagawa, Keiko Tamiya-Koizumi, Masnori Hattori, Motoshi Sawada, Yoshio Hirabayasi, Nobuko Ohishi, Yoshinori Nozawa

研究成果: Article

16 引用 (Scopus)

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We have investigated to determine the source of ceramide produced during the genotoxic apoptosis induced by the anti-cancer drug, camptothecin (CPT), in human prostate cancer LNCaP cells by measuring the activities of acid and neutral sphingomyelinases (SMase) and by using fumonisinB1 (FB1), the inhibitor of ceramide synthase involving de novo synthesis of ceramide. In contrast to time-dependent elevation of intracellular ceramide level after CPT-treatment, the activities of both SMases were not increased but rather decreased. Instead, pretreatment for 3 h with FB1 (100 μM), an inhibitor of ceramide synthase, almost completely abrogated ceramide accumulation observed in cells exposed to CPT for 18 h. These results indicate that ceramide is produced via de novo pathway but not via sphingomyelin hydrolysis pathway. Furthermore, it is to be noted that the pretreatment with FB1 did not affect the CPT-induced apoptosis as assessed by DNA ladder formation, Hoechst 33342 staining, flow cytometry, and mitochondrial potential thereby leading us to propose that ceramide accumulation is independent of apoptosis in this system.

元の言語English
ページ(範囲)363-370
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
294
発行部数2
DOI
出版物ステータスPublished - 01-01-2002
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Akao, Y., Kusakabe, S., Banno, Y., Kito, M., Nakagawa, Y., Tamiya-Koizumi, K., Hattori, M., Sawada, M., Hirabayasi, Y., Ohishi, N., & Nozawa, Y. (2002). Ceramide accumulation is independent of camptothecin-induced apoptosis in prostate cancer LNCaP cells. Biochemical and Biophysical Research Communications, 294(2), 363-370. https://doi.org/10.1016/S0006-291X(02)00462-X