Changes in extracellular nitrite and nitrate levels after inhibition of glial metabolism with fluorocitrate

Kiyofumi Yamada, Kouji Senzaki, Yumiko Komori, Toshiaki Nikai, Hisayoshi Sugihara, Toshitaka Nabeshima

研究成果: Article

13 引用 (Scopus)

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The role of glial cells in nitric oxide production in the cerebellum of conscious rats was investigated with a glial selective metabolic inhibitor, fluorocitrate. The levels of nitric oxide metabolites (nitrite plus nitrate) in the dialysate following in vivo microdialysis progressively increased to more than 2-fold the basal levels during a 2-h infusion of fluorocitrate (1 mM), and the increase persisted for more than 2 h after the treatment. Pretreatment with N(G)-nitro-L-arginine methyl ester attenuated the fluorocitrate-induced increase in nitric oxide metabolite levels. None of the glutamate receptor antagonists, including D(-)-2-amino-5-phosphonopentanoic acid, 6,7-dinitroquinoxaline-2,3-dione, and (±)-α-methyl-4- carboxyphenylglycine, inhibited the fluorocitrate-induced increase. The L- arginine-induced increase was significantly reduced by fluorocitrate treatment, while N-methyl-D-aspartate, (+)-α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid, and trans-(±)-1-amino-(1S,3R)- cyclopentane-dicarboxylic acid increased nitric oxide metabolites levels in the fluorocitrate-treated rats, as much as in control animals. These results suggest that glial cells play an important role in modulating nitric oxide production in the cerebellum by regulating L-arginine availability.

元の言語English
ページ(範囲)72-78
ページ数7
ジャーナルBrain Research
762
発行部数1-2
DOI
出版物ステータスPublished - 11-07-1997
外部発表Yes

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All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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