TY - JOUR
T1 - Characterization of a naturally occurring class D β-lactamase from Achromobacter xylosoxidans
AU - Doi, Yohei
AU - Poirel, Laurent
AU - Paterson, David L.
AU - Nordmann, Patrice
PY - 2008/6
Y1 - 2008/6
N2 - A chromosomally encoded class D β-lactamase, OXA-114, was characterized from Achromobacter xylosoxidans strain CIP69598. β-Lactamase OXA-114 shared 56% amino acid identity with the naturally occurring class D β-lactamase of Burkholderia cenocepacia and 42% identity with the acquired oxacillinases OXA-9 and OXA-18. OXA-114 has a narrow-spectrum hydrolysis profile, although it includes imipenem, at a very low level. PCR and sequencing revealed that blaOXA.114-like genes were identified in all A. xylosoxidans strains tested (n = 5), indicating that this β-lactamase is naturally occurring in that species. Induction experiments with imipenem and cefoxitin did not show inducibility of blaOXA.114 expression.
AB - A chromosomally encoded class D β-lactamase, OXA-114, was characterized from Achromobacter xylosoxidans strain CIP69598. β-Lactamase OXA-114 shared 56% amino acid identity with the naturally occurring class D β-lactamase of Burkholderia cenocepacia and 42% identity with the acquired oxacillinases OXA-9 and OXA-18. OXA-114 has a narrow-spectrum hydrolysis profile, although it includes imipenem, at a very low level. PCR and sequencing revealed that blaOXA.114-like genes were identified in all A. xylosoxidans strains tested (n = 5), indicating that this β-lactamase is naturally occurring in that species. Induction experiments with imipenem and cefoxitin did not show inducibility of blaOXA.114 expression.
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U2 - 10.1128/AAC.01463-07
DO - 10.1128/AAC.01463-07
M3 - Article
C2 - 18362192
AN - SCOPUS:44449177037
SN - 0066-4804
VL - 52
SP - 1952
EP - 1956
JO - Antimicrobial agents and chemotherapy
JF - Antimicrobial agents and chemotherapy
IS - 6
ER -
