Characterization of aged rat vocal fold fibroblasts

Yoshitaka Kawai, Yo Kishimoto, Tohru Sogami, Ryo Suzuki, Takuya Tsuji, Nao Hiwatashi, Ichiro Tateya, Shin Ichi Kanemaru, Tatsuo Nakamura, Koichi Omori, Shigeru Hirano

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Objectives/Hypothesis: To elucidate the aging physiology of the vocal folds, we examined the characters of aged vocal fold fibroblasts (VFFs) in various conditions. Study Design: In vitro study. Methods: VFFs from young (12-week-old) and aged (19-month-old) Sprague-Dawley rats were compared. Proliferative capacity, ratio of myofibroblast to fibroblast, myofibroblast function, and extracellular matrix production were examined in the following conditions: naïve, basic fibroblast growth factor (bFGF) supplemented, and hepatocyte growth factor (HGF) supplemented. Results: Aged VFFs demonstrated reduced proliferation by cell counting, though the ratio of Ki-67–positive cells showed no difference. Aged VFFs exhibited an increased expression of α-smooth muscle actin (α-SMA); however, they demonstrated no enhanced contractile ability in a gel contraction assay. Type I collagen protein was increased age dependently, accompanied with decreased Mmp1 and unchanged Col1a1 transcription. Type I collagen protein and α-SMA represented quite similar reduction patterns to bFGF or HGF administration. Conclusions: The following possible characteristics of aged VFFs were implied: long duration of mitosis, increased myofibroblast population size with certain dysfunctions, reduced type I collagen turnover, and correlation between α-SMA expression and type I collagen metabolism. Further investigations of these features will help to clarify presbyphonia's pathology and establish treatment strategies. Level of Evidence: NA Laryngoscope, 129:E94–E101, 2019.

元の言語English
ページ(範囲)E94-E101
ジャーナルLaryngoscope
129
発行部数3
DOI
出版物ステータスPublished - 03-2019
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

フィンガープリント Characterization of aged rat vocal fold fibroblasts' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Kawai, Y., Kishimoto, Y., Sogami, T., Suzuki, R., Tsuji, T., Hiwatashi, N., Tateya, I., Kanemaru, S. I., Nakamura, T., Omori, K., & Hirano, S. (2019). Characterization of aged rat vocal fold fibroblasts. Laryngoscope, 129(3), E94-E101. https://doi.org/10.1002/lary.27464