Characterization of biological activities of Brucella melitensis lipopolysaccharide

Gantsetseg Tumurkhuu; Naoki Koide; Kazuko Takahashi; Ferdaus Hassan; Shamima Islam; Hiroyasu Ito; Isamu Mori; Tomoaki Yoshida; Takashi Yokochi

doi:10.1111/j.1348-0421.2006.tb03810.x

Characterization of biological activities of Brucella melitensis lipopolysaccharide

Gantsetseg Tumurkhuu, Naoki Koide, Kazuko Takahashi, Ferdaus Hassan, Shamima Islam, Hiroyasu Ito, Isamu Mori, Tomoaki Yoshida, Takashi Yokochi

研究成果: Article › 査読

17 被引用数 (Scopus)

抄録

Biological activities of lipopolysaccharide (LPS) from Brucella melitensis 16M were characterized in comparison with LPS from Escherichia coli O55. LPS extracted from B. melitensis was smooth type by electrophoretic analysis with silver staining. The endotoxin-specific Limulus activity of B. melitensis LPS was lower than that of E. coli LPS. There was no significant production of tumor necrosis factor-α and nitric oxide in RAW 264.7 macrophage cells stimulated with B. melitensis LPS, although E. coli LPS definitely induced their production. On the other hand, B. melitensis LPS exhibited a higher anti-complement activity than E. coli LPS. B. melitensis LPS as well as E. coli LPS exhibited a strong adjuvant action on antibody response to bovine serum. The characteristic biological activities of B. melitensis are discussed.

本文言語	English
ページ（範囲）	421-427
ページ数	7
ジャーナル	MICROBIOLOGY and IMMUNOLOGY
巻	50
号	6
DOI	https://doi.org/10.1111/j.1348-0421.2006.tb03810.x
出版ステータス	Published - 2006
外部発表	はい

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Virology

文献へのアクセス

10.1111/j.1348-0421.2006.tb03810.x

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引用スタイル

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title = "Characterization of biological activities of Brucella melitensis lipopolysaccharide",

abstract = "Biological activities of lipopolysaccharide (LPS) from Brucella melitensis 16M were characterized in comparison with LPS from Escherichia coli O55. LPS extracted from B. melitensis was smooth type by electrophoretic analysis with silver staining. The endotoxin-specific Limulus activity of B. melitensis LPS was lower than that of E. coli LPS. There was no significant production of tumor necrosis factor-α and nitric oxide in RAW 264.7 macrophage cells stimulated with B. melitensis LPS, although E. coli LPS definitely induced their production. On the other hand, B. melitensis LPS exhibited a higher anti-complement activity than E. coli LPS. B. melitensis LPS as well as E. coli LPS exhibited a strong adjuvant action on antibody response to bovine serum. The characteristic biological activities of B. melitensis are discussed.",

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T1 - Characterization of biological activities of Brucella melitensis lipopolysaccharide

AU - Tumurkhuu, Gantsetseg

AU - Koide, Naoki

AU - Takahashi, Kazuko

AU - Hassan, Ferdaus

AU - Islam, Shamima

AU - Ito, Hiroyasu

AU - Mori, Isamu

AU - Yoshida, Tomoaki

AU - Yokochi, Takashi

PY - 2006

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AB - Biological activities of lipopolysaccharide (LPS) from Brucella melitensis 16M were characterized in comparison with LPS from Escherichia coli O55. LPS extracted from B. melitensis was smooth type by electrophoretic analysis with silver staining. The endotoxin-specific Limulus activity of B. melitensis LPS was lower than that of E. coli LPS. There was no significant production of tumor necrosis factor-α and nitric oxide in RAW 264.7 macrophage cells stimulated with B. melitensis LPS, although E. coli LPS definitely induced their production. On the other hand, B. melitensis LPS exhibited a higher anti-complement activity than E. coli LPS. B. melitensis LPS as well as E. coli LPS exhibited a strong adjuvant action on antibody response to bovine serum. The characteristic biological activities of B. melitensis are discussed.

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