TY - JOUR
T1 - Characterization of biological activities of Brucella melitensis lipopolysaccharide
AU - Tumurkhuu, Gantsetseg
AU - Koide, Naoki
AU - Takahashi, Kazuko
AU - Hassan, Ferdaus
AU - Islam, Shamima
AU - Ito, Hiroyasu
AU - Mori, Isamu
AU - Yoshida, Tomoaki
AU - Yokochi, Takashi
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2006
Y1 - 2006
N2 - Biological activities of lipopolysaccharide (LPS) from Brucella melitensis 16M were characterized in comparison with LPS from Escherichia coli O55. LPS extracted from B. melitensis was smooth type by electrophoretic analysis with silver staining. The endotoxin-specific Limulus activity of B. melitensis LPS was lower than that of E. coli LPS. There was no significant production of tumor necrosis factor-α and nitric oxide in RAW 264.7 macrophage cells stimulated with B. melitensis LPS, although E. coli LPS definitely induced their production. On the other hand, B. melitensis LPS exhibited a higher anti-complement activity than E. coli LPS. B. melitensis LPS as well as E. coli LPS exhibited a strong adjuvant action on antibody response to bovine serum. The characteristic biological activities of B. melitensis are discussed.
AB - Biological activities of lipopolysaccharide (LPS) from Brucella melitensis 16M were characterized in comparison with LPS from Escherichia coli O55. LPS extracted from B. melitensis was smooth type by electrophoretic analysis with silver staining. The endotoxin-specific Limulus activity of B. melitensis LPS was lower than that of E. coli LPS. There was no significant production of tumor necrosis factor-α and nitric oxide in RAW 264.7 macrophage cells stimulated with B. melitensis LPS, although E. coli LPS definitely induced their production. On the other hand, B. melitensis LPS exhibited a higher anti-complement activity than E. coli LPS. B. melitensis LPS as well as E. coli LPS exhibited a strong adjuvant action on antibody response to bovine serum. The characteristic biological activities of B. melitensis are discussed.
UR - https://www.scopus.com/pages/publications/33745245561
UR - https://www.scopus.com/pages/publications/33745245561#tab=citedBy
U2 - 10.1111/j.1348-0421.2006.tb03810.x
DO - 10.1111/j.1348-0421.2006.tb03810.x
M3 - Article
C2 - 16785713
AN - SCOPUS:33745245561
SN - 0385-5600
VL - 50
SP - 421
EP - 427
JO - MICROBIOLOGY and IMMUNOLOGY
JF - MICROBIOLOGY and IMMUNOLOGY
IS - 6
ER -