Characterization of murine CD160+ CD8+ T lymphocytes

Kunio Tsujimura, Yuichi Obata, Yasue Matsudaira, Keiko Nishida, Yoshiki Akatsuka, Yoshinori Ito, Ayako Demachi-Okamura, Kiyotaka Kuzushima, Toshitada Takahashi

研究成果: Article

34 引用 (Scopus)

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CD160 is an Ig-like glycoprotein expressed on NK, NKT and TCRγδ T cells, as well as intestinal intraepithelial T lymphocytes. In addition, a minor subset of CD8+ but not CD4+ T cells in the periphery is also known to express CD160, but the subset has not been fully characterized. In this study, we prepared anti-murine CD160 mAbs and investigated the expression profile of CD160 on various subsets of CD8+ T cells. The amount of CD160 on almost all CD8+ T cells was increased upon CD3-mediated stimulation in vitro, and soluble CD160 was found to be released. Flow cytometric analysis revealed most CD8+ T cells expressing CD160 to show a CD44high phenotype in vivo. On further analysis, both CD44highCD62Llow effector memory T cells (TEM) and CD44highCD62Lhigh central memory T cells (TCM) expressed CD160 at an intermediate level. High levels were evident with recently activated CD8+ TEM. Naïve CD8+ T cells presumably immediately after stimulation (CD44lowCD62LlowCD69+) also expressed CD160, but only at a low level. Purified CD160+ CD8+ T cells from OT-1 transgenic mice expressing TCR against OVA residues 257-264 presented by H-2Kb produced IFN-γ more rapidly than CD160- CD8+ T cells upon antigen stimulation. These results together show that CD160 is expressed on the majority of CD8+ memory T cells as well as recently activated CD8+ T cells.

元の言語English
ページ(範囲)48-56
ページ数9
ジャーナルImmunology Letters
106
発行部数1
DOI
出版物ステータスPublished - 15-07-2006

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

フィンガープリント Characterization of murine CD160<sup>+</sup> CD8<sup>+</sup> T lymphocytes' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Tsujimura, K., Obata, Y., Matsudaira, Y., Nishida, K., Akatsuka, Y., Ito, Y., Demachi-Okamura, A., Kuzushima, K., & Takahashi, T. (2006). Characterization of murine CD160+ CD8+ T lymphocytes. Immunology Letters, 106(1), 48-56. https://doi.org/10.1016/j.imlet.2006.04.006