Characterization of spontaneous excitatory synaptic currents in newt retinal bipolar cells

研究成果: Article査読

5 被引用数 (Scopus)

抄録

The kinetics of glutamate concentration in the synaptic cleft is an important determinant of synaptic function. To elucidate peak concentration of glutamate released from a single vesicle in the cleft, spontaneous excitatory postsynaptic currents (sEPSCs)in Off-bipolar cells from the sliced newt retina were analyzed using whole-cell patch clamp recording and the computer simulation. The sEPSCs were blocked by an AMPA/kainate (KA) antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), and prolonged by cyclothiazide. However, an N-methyl-D-aspartate (NMDA) antagonist, D-2- amino-5-phosphonopentanoic acid (D-AP5), was ineffective. These suggest that sEPSCs in Off-bipolar cells are mediated exclusively by AMPA/KA receptors, sEPSCs simulated by a detailed kinetic model of AMPA receptor best approximated the data, when peak glutamate concentration was 10 μM. Therefore, it was concluded that peak concentration of glutamate released from a single vesicle would be elevated to approximately 10 μM at the newt Off-bipolar dendrite.

本文言語English
ページ(範囲)49-52
ページ数4
ジャーナルNeuroscience Letters
271
1
DOI
出版ステータスPublished - 13-08-1999
外部発表はい

All Science Journal Classification (ASJC) codes

  • 神経科学(全般)

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