Characterization of the neurotoxin produced by isolates associated with avian botulism

Masato Takeda, Kentaro Tsukamoto, Tomoko Kohda, Miki Matsui, Masafumi Mukamoto, Shunji Kozaki

研究成果: Article査読

68 被引用数 (Scopus)


Several varieties of birds are affected by type C botulism. We conducted neutralization tests of culture supernatants of isolates from cases of avian botulism. Whereas the toxin produced by isolates derived from mammalian botulism was neutralized only with type C antitoxin, the toxins of all isolates related to avian botulism were neutralized with both type C and D antitoxins. An analysis of nucleotide sequences with several strains revealed that the neurotoxin gene in the isolates from avian botulism comprises two thirds of the type C neurotoxin gene and one third of the type D neurotoxin gene. This indicates that the neurotoxin of avian isolates is a mosaic of type C and D neurotoxins. We prepared three sets of primers to differentiate the gene for the mosaic form from the conserved genes of type C and D neurotoxins. The results of polymerase chain reaction with these primers indicated that all avian botulism-related isolates and specimens possess the gene for the mosaic form of the neurotoxin. The toxins purified from avian and mammalian isolates exhibited the same degree of lethality in mice, but the former showed greater toxicity to chickens than the latter. These results indicate that the mosaic neurotoxin is probably a pathogenic agent causing some forms of avian botulism.

ジャーナルAvian Diseases
出版ステータスPublished - 09-2005

All Science Journal Classification (ASJC) codes

  • 食用動物
  • 動物科学および動物学
  • 免疫学および微生物学(全般)


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