Characterization of the promoter region of the human RFP gene

Yosuke Iwata, Atsuo Nakayama, Hideki Murakami, Ken Ichi Iida, Toshihide Iwashita, Naoya Asai, Masahide Takahashi

研究成果: Article査読

8 被引用数 (Scopus)

抄録

The RFP gene encodes a Ring finger protein that has a tripartite motif consisting of a Ring finger, a B-box finger and a coiled-coil domain. In the present study, we cloned and characterized the promoter region of the human RFP gene. The nucleotide sequence of the promoter was GC-rich and had no typical TATA and CAAT boxes. Instead, it contained one AP2 and two Sp1 binding sites within 100 base pairs upstream of the transcription initiation site. Analysis by the luciferase assay revealed that the activity of this promoter region is very strong in both human and mouse cell lines, although the activity in human cells was approximately 10-15 fold higher than that in mouse cells. In addition, the AP2 and Sp1 binding sites appeared to synergistically function for the promoter activity. Thus, the promoter of the RFP gene could be useful for high levels of expression of various genes in culture cells.

本文言語English
ページ(範囲)381-384
ページ数4
ジャーナルBiochemical and Biophysical Research Communications
261
2
DOI
出版ステータスPublished - 02-08-1999
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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