TY - JOUR
T1 - Characterization of the promoter region of the human RFP gene
AU - Iwata, Yosuke
AU - Nakayama, Atsuo
AU - Murakami, Hideki
AU - Iida, Ken Ichi
AU - Iwashita, Toshihide
AU - Asai, Naoya
AU - Takahashi, Masahide
N1 - Funding Information:
We thank Mr. Imaizumi for technical assistance. This work was supported in part by grants-in-aid for COE research, Scientific Research, and Cancer Research from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 1999/8/2
Y1 - 1999/8/2
N2 - The RFP gene encodes a Ring finger protein that has a tripartite motif consisting of a Ring finger, a B-box finger and a coiled-coil domain. In the present study, we cloned and characterized the promoter region of the human RFP gene. The nucleotide sequence of the promoter was GC-rich and had no typical TATA and CAAT boxes. Instead, it contained one AP2 and two Sp1 binding sites within 100 base pairs upstream of the transcription initiation site. Analysis by the luciferase assay revealed that the activity of this promoter region is very strong in both human and mouse cell lines, although the activity in human cells was approximately 10-15 fold higher than that in mouse cells. In addition, the AP2 and Sp1 binding sites appeared to synergistically function for the promoter activity. Thus, the promoter of the RFP gene could be useful for high levels of expression of various genes in culture cells.
AB - The RFP gene encodes a Ring finger protein that has a tripartite motif consisting of a Ring finger, a B-box finger and a coiled-coil domain. In the present study, we cloned and characterized the promoter region of the human RFP gene. The nucleotide sequence of the promoter was GC-rich and had no typical TATA and CAAT boxes. Instead, it contained one AP2 and two Sp1 binding sites within 100 base pairs upstream of the transcription initiation site. Analysis by the luciferase assay revealed that the activity of this promoter region is very strong in both human and mouse cell lines, although the activity in human cells was approximately 10-15 fold higher than that in mouse cells. In addition, the AP2 and Sp1 binding sites appeared to synergistically function for the promoter activity. Thus, the promoter of the RFP gene could be useful for high levels of expression of various genes in culture cells.
UR - https://www.scopus.com/pages/publications/0033517039
UR - https://www.scopus.com/pages/publications/0033517039#tab=citedBy
U2 - 10.1006/bbrc.1999.1037
DO - 10.1006/bbrc.1999.1037
M3 - Article
C2 - 10425194
AN - SCOPUS:0033517039
SN - 0006-291X
VL - 261
SP - 381
EP - 384
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -